From 2007.igem.org

Revision as of 03:24, 27 October 2007 (view source) Maiko (Talk | contribs) (→Design) ← Older edit		Revision as of 03:31, 27 October 2007 (view source) Maiko (Talk | contribs) (→Making Affinity Tags) Newer edit →
Line 37:		Line 37:
	==Achievements Overview==		==Achievements Overview==
	===Making Affinity Tags===		===Making Affinity Tags===
-	#Tagとなるヒスチジンペプチドが入った鞭毛をつくる．	+	#Make the affinity tag by inserting the his-tag into the flagellar fillament.
-	#*発現をチェック	+	#*[[Image:Chiba_check.png]] Sequence confirmed
-	#*ディスプレイをチェック	+	#*[[Image:Chiba_check.png]] Swarm confirmed
		+	#*[[Image:Chiba_check.png]] Flagella strained with anti-flagella antibody
		+	#*[[Image:Chiba_check.png]] Phenotype confirmed
		+	#*[[Image:Chiba_check.png]] Affnity confirmed (see the figure below.)
		+
	===Making Communication Module===		===Making Communication Module===
	#Making Senders		#Making Senders

---

Revision as of 03:31, 27 October 2007

Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿

Chiba's Project

Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?) Motivation.
(more...)

Design

Our marimo bacteria requres as follows:

	1.Affinity Tag. Make a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.
	2.Communication Module. Make a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.
	3.Size Controler. Make an AHL localized region for quorum sensing.

これを組み合わせて以下のように毬藻をつくる：

Achievements Overview

Making Affinity Tags

1. Make the affinity tag by inserting the his-tag into the flagellar fillament.
   • Sequence confirmed
   • Swarm confirmed
   • Flagella strained with anti-flagella antibody
   • Phenotype confirmed
   • Affnity confirmed (see the figure below.)

Making Communication Module

1. Making Senders
   • 1.---
   • 2.---
2. Making Receivers
   • 1.---
   • 2.---

Controlling Size

1. Improve Sender
   • 前駆体合成酵素metKを入れてみた．＞結果？
2. Improve Receiver
   • LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
3. Localize AHL
   • aiiAその１の回路（〜〜になるはず）＞結果
   • aiiAその２の回路
   • その３

Making Marimo

Remaining Task(Work)

Our Goal

Future