MIT

From 2007.igem.org

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*Please see our '''[http://openwetware.org/wiki/IGEM:MIT/2007 wiki]''' on OpenWetWare for our official website and more information!  This here is a summary of our project.
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Mercury contamination of drinking water is a significant problem in both developed and developing countries. Techniques to filter it out are both costly and intensive. Thus, the MIT iGEM 2007 team is engineering a biological mechanism to cost-effectively sense and remove Mercury ions from contaminated water through a two cell system. One cell will use the Mer promoter to sense the presence of Mercury ions, then activate the GFP fused downstream. The other uses a cell surface display mechanism to exhibit a Mercury capturing peptide, extracting the Mercury from the water. Both cells also display polystyrene binding peptides, and will thus be attached to a polystyrene filter. This setup would be easy to use, cheap to manufacture, and economical to distribute. It could be used from very small scales to even an entire village's drinking water supply.
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Resources
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*[http://openwetware.org/wiki/IGEM:MIT/2007/Notebook Team Notebook]
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*[[/Background|Background]]
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*[[/Approach|Approach]]
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*[[/Results|Results]]
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*[[/Future|Future Experiments]]
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'''Team Detox''' presents '''Project E.coFilter''' 
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<!--*[[Team Notebook]]-->
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*'''Brainstorming''':
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**[http://openwetware.org/wiki/IGEM:MIT/2007/Heavy_Metals Heavy Metals]
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**[http://openwetware.org/wiki/IGEM:MIT/2007/Signalers Signalers]
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**[http://openwetware.org/wiki/IGEM:MIT/2007/Links_for_Bioremediation_Project Links for Bioremediation Project]
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**[http://openwetware.org/wiki/IGEM:MIT/2007/Ideas Ideas]
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**[http://openwetware.org/wiki/IGEM:MIT/2007/UpdatedIdeas Updated Ideas]
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**[http://openwetware.org/wiki/IGEM:MIT/2007/Ideas3 Final Discussion]
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**[http://del.icio.us/MITiGEM Background reading]
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We are using surface display of polystyrene-binding proteins and heavy metal promoters to sense and remove Hg ions from contaminated water.
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===External links===
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*[http://www.openwetware.org/wiki/iGEM iGEM on OpenWetWare]
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*[http://partsregistry.org Parts registry]
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*Our Parts:
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**[http://partsregistry.org/Part:BBa_I728005 OmpC Surface Display]
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**[http://partsregistry.org/Part:BBa_I728004 CPX Surface Display]
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===For visitors===
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*Read more about [[Synthetic Biology|engineering biology]].
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*[[../2006|2006 MIT iGEM wiki]]
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*[[../2005/|2005 MIT iGEM wiki]]
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*[[Synthetic_Biology:Polka_Dots|Polka Dots]]: The MIT IAP class, a precursor to iGEM
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===Internal links===
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*'''[[../2007/Notebook|Team Notebook]]'''
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*'''[[/Biobricks|Biobricks]]'''
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*'''[[/Red_Team|Red Team]]'''
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*'''[[/Contacts|Contact Numbers]]'''
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*'''[[BE.109:DNA_engineering|DNA engineering from BE.109]]'''
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*'''[[/Protocols|Protocols]]'''
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*'''[[/Nomenclature|Nomenclature and Abbreviations]]'''
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*'''[[/Codes|Test Tube Codes]]'''
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*'''[[/OrderInfo|Order Information]]'''
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*'''[[/Inventory|Inventory]]'''
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[[Image:Wholeoverview.jpg|thumb|center|300px|center|MIT's Project Goal: Sensing and Removing Hg ions from contaminated water.]]
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=== Want to join the team? ===
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[[Image:IGEM_team.jpg|thumb|center|300px|Top Row: Forrest Liau, Aditya Kohli, Brian Cook, Alex Lue, Semmie Kim, Bernice Huang, Jess Ho.  Bottom Row: Debbie Liu, Laure-Anne Ventouras, Toan Tran-Phu]]
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*'''Graduate''' students interested in advising the team should email '''grads [AT] igem.mit.edu'''
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*'''Undergraduate''' students will have to wait until 2008!
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===Schedule===
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*April 8 - Application deadline (5pm on Sunday)
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*April 7-9 - Undergrad interviews
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*April 12 - UROP funding deadline
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*April 30 - iGEM 2007 registration closes
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*May 1 - Team 2-page proposals due
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*May 15 - Preliminary team rosters due
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*May 26 - Teach the teachers workshop
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*May 30 - Team registration fee due
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*June 4 - Team training begins
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*June 11 - Team starts in the lab (summer term begins)
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*July 1 - Team project descriptions due
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*September 1 - Final team rosters due
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*October 26 - Project and part documentation due
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*October 26 - BioBrick Part DNA received by the Registry
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*November 3-4 - iGEM competition Jamboree, MIT, USA
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<img src="http://partsregistry.org/wiki/images/d/db/Buttonaddpart.gif" width=20>
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Today = new Date();
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Jamboree = new Date("November 3, 2007");
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msInADay = 1000 * 60 * 60 * 24;
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display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay);
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document.write("There are " + display +" days left until the Jamboree!");
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BioBrick Parts
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----
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*[http://partsregistry.org/Part:BBa_I728005 OmpC Surface Display]
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===Brainstorming===
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*[http://partsregistry.org/Part:BBa_I728004 CPX Surface Display]
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*'''[[/Heavy Metals|Heavy Metals]]'''
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*[http://partsregistry.org/Part:BBa_I728500 CPX display of a polystyrene binding peptide]
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*'''[[/Signalers|Signalers]]'''
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*[http://partsregistry.org/Part:BBa_I728200 Simple System of Transcriptional Regulator, RBS, and CPX Surface Display Mechanism]
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*'''[[/Links for Bioremediation Project|Links for Bioremediation Project]]'''
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*[http://partsregistry.org/Part:BBa_I728201 Complex System of Promoter, RBS, CPX, and terminator]
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*'''[[/Ideas|Ideas]]'''
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*'''[[/UpdatedIdeas|Updated Ideas]]'''
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*'''[[/Ideas3|Final Discussion]]'''
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*'''[http://del.icio.us/MITiGEM Background reading]'''
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===Meeting Logistics===
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*'''[[/Room Reservations|Room Reservations]]'''
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*'''[[/Onduty|Gradvisor "On Duty" Shifts]]'''
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===Last year's MIT iGEM contact info===
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*Email grads: '''grads06 [AT] igem.mit.edu'''
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*Email team: '''smellers [AT] igem.mit.edu'''
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The MIT iGEM team consists of six undergraduate students working fulltime during summer 2007 on engineering a biological system.  In addition, we have a number of graduate student and faculty advisors.
 
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<!---'''New: [[Special:Chat/IGEM:MIT/2007| iGEM chat room!]]'''-->
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'''[[Media:MITiGEM.jpg|Picture of MIT iGEM 2007]]'''
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====Students====
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*[http://openwetware.org/wiki/User:Jessho Jessica Ho]
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*[http://openwetware.org/wiki/User:Bernice Bernice Huang]
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*[http://openwetware.org/wiki/User:Awlue Alexander Lue]
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*[http://openwetware.org/wiki/User:Skim Semmie Kim]
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*[http://openwetware.org/wiki/User:Akohli Aditya Kohli]
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*[http://openwetware.org/wiki/User:Toan Toan Tran-Phu]
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===Students===
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Email us: '''team [AT] igem.mit.edu'''
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*[[User:Jessho|Jessica Ho]]
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*[[User:Bernice|Bernice Huang]]
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*[[User:Awlue|Alexander Lue]]
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*[[User:Skim|Semmie Kim]]
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*[[User:akohli|Aditya Kohli]]
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*[[User:Toan|Toan Tran-Phu]]
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Email us: '''team [AT] igem.mit.edu'''
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====Advisors====
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*[http://openwetware.org/wiki/User:Anton1 Anton Aboukhalil]
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*[http://openwetware.org/wiki/User:Barbero Robbie Barbero]
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*[http://openwetware.org/wiki/User:Carr Peter Carr]
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*[http://openwetware.org/wiki/User:Cookb Brian Cook]
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*[http://openwetware.org/wiki/Drew_Endy Drew Endy]
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*[http://openwetware.org/wiki/User:Ghoshd Rana Ghosh]
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*[http://openwetware.org/wiki/User:Hyungdo Hyung-Do Kim]
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*[http://openwetware.org/wiki/Tom_Knight Tom Knight]
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*[http://openwetware.org/wiki/User:Krauland Eric Krauland]
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*[http://openwetware.org/wiki/Forrest_Liau Forrest Liau]
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*[http://openwetware.org/wiki/Debbie_Liu Debbie Liu]
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*[http://openwetware.org/wiki/User:Ryrolitt Romie Littrell]
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*[http://openwetware.org/wiki/User:Venkataramanan Venkataramanan Soundararajan]
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*[http://openwetware.org/wiki/User:Lventouras Laure-Anne Ventouras]
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===Advisors===
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Email grads: '''grads [AT] igem.mit.edu'''
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*[[User:Anton1|Anton Aboukhalil]]
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Email all: '''igem [AT] igem.mit.edu'''
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*[[User:Barbero|Robbie Barbero]]
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*[[User:Carr|Peter Carr]]
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*[[User:Cookb|Brian Cook]]
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*[[Drew Endy]]
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*[[User:Ghoshd|Rana Ghosh]]
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*[[User:Hyungdo|Hyung-Do Kim]]
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*[[Tom Knight]]
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*[[User:Krauland|Eric Krauland]]
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*[[Forrest Liau|Forrest Liau]]
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*[[Debbie Liu|Debbie Liu]]
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*[[User:Ryrolitt|Romie Littrell]]
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*[[User:Venkataramanan|Venkataramanan Soundararajan]]
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*[[User:Lventouras|Laure-Anne Ventouras]]
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Email grads: '''grads [AT] igem.mit.edu'''
 
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Email all: '''igem [AT] igem.mit.edu'''
 
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Latest revision as of 03:46, 27 October 2007

  • Please see our [http://openwetware.org/wiki/IGEM:MIT/2007 wiki] on OpenWetWare for our official website and more information! This here is a summary of our project.

Information Information

Mercury contamination of drinking water is a significant problem in both developed and developing countries. Techniques to filter it out are both costly and intensive. Thus, the MIT iGEM 2007 team is engineering a biological mechanism to cost-effectively sense and remove Mercury ions from contaminated water through a two cell system. One cell will use the Mer promoter to sense the presence of Mercury ions, then activate the GFP fused downstream. The other uses a cell surface display mechanism to exhibit a Mercury capturing peptide, extracting the Mercury from the water. Both cells also display polystyrene binding peptides, and will thus be attached to a polystyrene filter. This setup would be easy to use, cheap to manufacture, and economical to distribute. It could be used from very small scales to even an entire village's drinking water supply.

Resources

  • Brainstorming:
    • [http://openwetware.org/wiki/IGEM:MIT/2007/Heavy_Metals Heavy Metals]
    • [http://openwetware.org/wiki/IGEM:MIT/2007/Signalers Signalers]
    • [http://openwetware.org/wiki/IGEM:MIT/2007/Links_for_Bioremediation_Project Links for Bioremediation Project]
    • [http://openwetware.org/wiki/IGEM:MIT/2007/Ideas Ideas]
    • [http://openwetware.org/wiki/IGEM:MIT/2007/UpdatedIdeas Updated Ideas]
    • [http://openwetware.org/wiki/IGEM:MIT/2007/Ideas3 Final Discussion]
    • [http://del.icio.us/MITiGEM Background reading]

Photos Graphics

MIT's Project Goal: Sensing and Removing Hg ions from contaminated water.
Top Row: Forrest Liau, Aditya Kohli, Brian Cook, Alex Lue, Semmie Kim, Bernice Huang, Jess Ho. Bottom Row: Debbie Liu, Laure-Anne Ventouras, Toan Tran-Phu

BioBrick Parts

  • [http://partsregistry.org/Part:BBa_I728005 OmpC Surface Display]
  • [http://partsregistry.org/Part:BBa_I728004 CPX Surface Display]
  • [http://partsregistry.org/Part:BBa_I728500 CPX display of a polystyrene binding peptide]
  • [http://partsregistry.org/Part:BBa_I728200 Simple System of Transcriptional Regulator, RBS, and CPX Surface Display Mechanism]
  • [http://partsregistry.org/Part:BBa_I728201 Complex System of Promoter, RBS, CPX, and terminator]

People People

Students

  • [http://openwetware.org/wiki/User:Jessho Jessica Ho]
  • [http://openwetware.org/wiki/User:Bernice Bernice Huang]
  • [http://openwetware.org/wiki/User:Awlue Alexander Lue]
  • [http://openwetware.org/wiki/User:Skim Semmie Kim]
  • [http://openwetware.org/wiki/User:Akohli Aditya Kohli]
  • [http://openwetware.org/wiki/User:Toan Toan Tran-Phu] 
Email us: team [AT] igem.mit.edu

Advisors

  • [http://openwetware.org/wiki/User:Anton1 Anton Aboukhalil]
  • [http://openwetware.org/wiki/User:Barbero Robbie Barbero]
  • [http://openwetware.org/wiki/User:Carr Peter Carr]
  • [http://openwetware.org/wiki/User:Cookb Brian Cook]
  • [http://openwetware.org/wiki/Drew_Endy Drew Endy]
  • [http://openwetware.org/wiki/User:Ghoshd Rana Ghosh]
  • [http://openwetware.org/wiki/User:Hyungdo Hyung-Do Kim]
  • [http://openwetware.org/wiki/Tom_Knight Tom Knight]
  • [http://openwetware.org/wiki/User:Krauland Eric Krauland]
  • [http://openwetware.org/wiki/Forrest_Liau Forrest Liau]
  • [http://openwetware.org/wiki/Debbie_Liu Debbie Liu]
  • [http://openwetware.org/wiki/User:Ryrolitt Romie Littrell]
  • [http://openwetware.org/wiki/User:Venkataramanan Venkataramanan Soundararajan]
  • [http://openwetware.org/wiki/User:Lventouras Laure-Anne Ventouras] 
Email grads: grads [AT] igem.mit.edu
Email all: igem [AT] igem.mit.edu



Retrieved from "http://2007.igem.org/wiki/index.php/MIT"