McGill

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<center>The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada</center><br>
<center>The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada</center><br>
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== Project Overview ==
 
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<u>'''Quorum-sensing coupled with the Repressilator'''</u>
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==  ==
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Our project is a continuation of one of the projects we presented last year: a two-gene oscillator, with an 'On' switch - LuxI gene, and an 'Off' switch - LacI, with a Cyan fluorescent protein bound, for visualization. This system works via the method of quorum-sensing between the two genes, with a diffusable artificial inducer (AI) protein produced by LuxI which couples when produced, to a constitutive gene in the system, LuxR to bind to LacI to turn the  system off. Once AI is produced, it can spread to other cells and continue this 'On'-'Off' oscillator in other neighbouring cells, and hence increasing synchronization across a population of genetically oscillating cells.
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This year we've taken this system even further by adding several elements of control to see how they effect oscillations:
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#By changing cell densities and observing their effect on the oscillator.
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#Adding an AI Analog (AHL) into the system.
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#Adding Tetracycline (DOX), an inhibitor of the LuxR promoter, to the system and observing its effect.
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#Adding an AI inhibitor (Aiia) into the system to control the levels of AI produced. <br>
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A major enhancement to the system this year is that we plan on adding the Ojalvo, Elowitz et al. - 'Represillator' system coupled to the two-gene oscillator in a single cell. The Repressilator is a 3-gene repressing network, where each gene represses the other, and so on. In turn, this system produces very clear oscillations but is hindered by noise and instability. It is theoried that this system can become highly stabilized if coupled with the 2-gene quorum-sensing oscillator, and once working side-by-side, the 2 systems will produce highly stable, smooth, sinusoidal oscillations.
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Both the quorum-sensing Oscillator and the Repressilator, though observed in a micro scale in our system, are important in helping understand time-varying conditions in the form of extrinsic driving from the environment and intrinsic rhythms generated within an organism itself. This includes specialized rhythm generators functioning in a coherent oscillatory state such as the cardiac pacemaker, also known as the sinoatrial node in mammalian hearts, and the circadian clock residing at the suprachiasmatic nuclei in mammalian brains. <br>
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<li><a href="https://2007.igem.org/McGill/Project_Description">Project</a></li>
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<li><a href="https://2007.igem.org/McGill/Modeling_and_Simulations">Simulations</a></li>
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<li><a href="https://2007.igem.org/McGill/Team_2_More_Information">Animations</a></li>
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<li><a href="https://2007.igem.org/McGill/Project_Overview">Future</a></li>
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<li><a href="https://2007.igem.org/McGill/Results">Results</a></li>
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<li><a href="https://2007.igem.org/McGill/Parts">Parts</a></li>
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[[McGill/Project Description|Project Description]]<br>
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== Project Overview ==
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[[McGill/Team_2_More_Information|More Information]] <br>
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[[McGill/Team_2_Background_Papers|Background Papers]] <br>
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<u>'''Quorum-sensing coupled with the Repressilator'''</u>
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<center>Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]</center><br>
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Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.
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Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.<br>
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==In the Lab==
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==[[Image:bacteria1.jpg]] In the Lab==
[[McGill/Lab Notebook|Lab Notebook]]<br>
[[McGill/Lab Notebook|Lab Notebook]]<br>
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[[McGill/Lab Protocols|Lab Protocols]]
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[[McGill/Lab Protocols|Lab Protocols]]<br>
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[[McGill/Team_2_Background_Papers|Background Papers]] <br>
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== The Team ==
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[[McGill/Team Roster| Team Roster]]<br>
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==[[Image:greenbulb.jpg]] The Team ==
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[[McGill/Team Roster| Team Members]]<br>
[[McGill/Mailing List|Mailing List]]
[[McGill/Mailing List|Mailing List]]
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==[[Image:sine.jpg]] Pictures ==
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[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup]  (External) <br>
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[[McGill/RI meetup|Rhode Island Meetup]]<br>
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[[McGill/urc|McGill Research Conference]]<br>
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Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]

Latest revision as of 03:57, 27 October 2007

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The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada

Contents

McGillIgem2007team.jpg






Project Overview

Quorum-sensing coupled with the Repressilator

Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.

Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.

2gene oscillator.jpg



Bacteria1.jpg In the Lab

Lab Notebook
Lab Protocols
Background Papers


Greenbulb.jpg The Team

Team Members
Mailing List

Sine.jpg Pictures

[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup] (External)
Rhode Island Meetup
McGill Research Conference

Please feel free to check out our work from last year at iGEM McGill 2006

Retrieved from "http://2007.igem.org/wiki/index.php/McGill"