McGill

From 2007.igem.org

(Difference between revisions)

Latest revision as of 03:57, 27 October 2007

The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada

Project Overview

Quorum-sensing coupled with the Repressilator

Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.

Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.

In the Lab

Lab Notebook
Lab Protocols
Background Papers

The Team

Team Members
Mailing List

Pictures

[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup] (External)
Rhode Island Meetup
McGill Research Conference

Please feel free to check out our work from last year at iGEM McGill 2006

@@ Line 1: / Line 1: @@
 [[Image:banner_1.gif]]
-<center>This is the official wiki of the McGill University iGEM 2007 team from Montreal, Quebec, Canada </center>
-== Project Overviews ==
-'''Team 1 - Fluorescence Complimentation'''
+<center>The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada</center><br>
+{| cellspacing="2px" cellpadding="5" border="0" style="padding: 0; width: 935px; color: #000000; background-color: #ffffff;"
+| width=750px style="padding: 20px; background-color: #ffffff; border: 4px solid #FF0000;" |
-This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence.  We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence.  These half proteins will then be attached to proteins that revesibly associate.<br>
+==  ==
+<center>[[Image:McGillIgem2007team.jpg|center|400px]]
+<html>
+<style>
-[[McGill/Team_1_More_Information|More Information]] <br>
+ul#list-nav {
-[[McGill/Team_1_Background_Papers|Background Papers]] <br>
+list-style:none;
+margin:20px;
+padding:0;
+}
-'''Team 2 - Repressilator'''
+ul#list-nav li {
+display:inline
+}
-Our project consists of a two-gene oscillator with coupling by an Autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations at different cell densities.<br>
+ul#list-nav li a {
+text-decoration:none;
+padding:10px 0;
+width:130px;
+background:#DB231E;
+color:#eee;
+float:left;
+text-align:center;
+border-left:1px solid #fff;
+}
-[[McGill/Team_2_More_Information|More Information]] <br>
+ul#list-nav li a:hover {
-[[McGill/Team_2_Background_Papers|Background Papers]] <br>
+background:#FFFFFF;
+color:#000
+}
+</style>
+<br>
+ <ul id="list-nav">
+<li><a href="https://2007.igem.org/McGill/Project_Description">Project</a></li>
+<li><a href="https://2007.igem.org/McGill/Modeling_and_Simulations">Simulations</a></li>
+<li><a href="https://2007.igem.org/McGill/Team_2_More_Information">Animations</a></li>
+<li><a href="https://2007.igem.org/McGill/Project_Overview">Future</a></li>
+<li><a href="https://2007.igem.org/McGill/Results">Results</a></li>
+<li><a href="https://2007.igem.org/McGill/Parts">Parts</a></li>
+</ul>
+</html>
+</center><br><br><br><br>
+== Project Overview ==
-Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]<br>
+<u>'''Quorum-sensing coupled with the Repressilator'''</u>
+Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.
-== In the Lab ==
+Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.<br>
+<center>
+[[Image:2gene oscillator.jpg|320px]]<br>
+</center><br>
+<br>
+{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
+|-valign="top"
+|width=240px style="padding: 5px|
+==[[Image:bacteria1.jpg]] In the Lab==
 [[McGill/Lab Notebook|Lab Notebook]]<br>
-[[McGill/Lab Protocols|Lab Protocols]]
+[[McGill/Lab Protocols|Lab Protocols]]<br>
+[[McGill/Team_2_Background_Papers|Background Papers]] <br>
-== The Team ==
-[[McGill/Team Roster| Team Roster]]<br>
-[[McGill/Mailing List|Mailing List]]
-== Important Notes ==
+|width=240px style="padding: 5px|
-When you make the links for the wiki pages, please follow this example:
+==[[Image:greenbulb.jpg]] The Team ==
+[[McGill/Team Roster| Team Members]]<br>
+[[McGill/Mailing List|Mailing List]]
-'''<nowiki>[[McGill/Link_to_page|Display Name]]</nowiki>'''
+|width=240px style="padding: 5px|
-An actual link would look like this in wiki code:
+==[[Image:sine.jpg]] Pictures ==
-'''<nowiki>[[McGill/Team_1:_Fluorescence_Complementation|Team 1: Fluorescence Complementation]]</nowiki>'''
+[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup]   (External) <br>
+[[McGill/RI meetup|Rhode Island Meetup]]<br>
+[[McGill/urc|McGill Research Conference]]<br>
-Thanks! - '''Hanmo'''
+Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]

Views

Personal tools