McGill

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Latest revision as of 03:57, 27 October 2007

The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada

Project Overview

Quorum-sensing coupled with the Repressilator

Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.

Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.

In the Lab

Lab Notebook
Lab Protocols
Background Papers

The Team

Team Members
Mailing List

Pictures

[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup] (External)
Rhode Island Meetup
McGill Research Conference

Please feel free to check out our work from last year at iGEM McGill 2006

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-<center>[[Image:McGillIgem2007team.jpg]]</center>
-== Project Overview ==
+==  ==
+<center>[[Image:McGillIgem2007team.jpg|center|400px]]
+<html>
+<style>
-<u>'''Quorum-sensing coupled with the Repressilator'''</u>
+ul#list-nav {
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-Our project is a continuation of one of the projects we presented last year: a two-gene oscillator, with an 'On' switch - LuxI gene, and an 'Off' switch - LacI, expressing a Cyan fluorescent protein when turned on for visualization. This simple relaxation oscillator makes a robust system of oscillations that can act as a 'pace maker' for more complicated oscillating systems. Quorum-sensing coupling between the systems is achieved with a diffusible artificial inducer (AI) protein made from LuxI which couples when produced, to a constitutively expressed gene in the system LuxR which can turn 'on' the system by promoting the synthesis of Lac from pLux. As Lac accumulates, it acts to turn 'off' the system by repressing the pLac controlling the synthesis of LuxI itself. Also, once AI is produced, it can easily diffuse to other cells and continue this 'On'-'Off' oscillator in other neighbouring cells, and hence increasing synchronization across a population of oscillating cells. <br>
+}
-This year we've taken this system even further...  <u>[[McGill/Project Overview|learn more]]</u><br><br>
+ul#list-nav li {
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-[[McGill/Project Description|Project Description]]<br>
+ul#list-nav li a {
-[[McGill/Team_2_More_Information|Animated System]] <br>
+text-decoration:none;
-[[McGill/Team_2_Background_Papers|Background Papers]] <br><br>
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-[[Image:repress_graph.jpg]]</center>
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+<br>
+ <ul id="list-nav">
+<li><a href="https://2007.igem.org/McGill/Project_Description">Project</a></li>
+<li><a href="https://2007.igem.org/McGill/Modeling_and_Simulations">Simulations</a></li>
+<li><a href="https://2007.igem.org/McGill/Team_2_More_Information">Animations</a></li>
+<li><a href="https://2007.igem.org/McGill/Project_Overview">Future</a></li>
+<li><a href="https://2007.igem.org/McGill/Results">Results</a></li>
+<li><a href="https://2007.igem.org/McGill/Parts">Parts</a></li>
+</ul>
+</html>
+</center><br><br><br><br>
-<center>Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]</center><br>
+== Project Overview ==
+<u>'''Quorum-sensing coupled with the Repressilator'''</u>
+Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.
+Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.<br>
+<center>
+[[Image:2gene oscillator.jpg|320px]]<br>
+</center><br>
+<br>
 {| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
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 [[McGill/Lab Notebook|Lab Notebook]]<br>
 [[McGill/Lab Protocols|Lab Protocols]]<br>
-[[McGill/Parts|Parts/Biobricks]]<br>
+[[McGill/Team_2_Background_Papers|Background Papers]] <br>
-[[McGill/Project Evaluation|Project Evaluation]]
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 [[McGill/RI meetup|Rhode Island Meetup]]<br>
 [[McGill/urc|McGill Research Conference]]<br>
-[[McGill/Team pics|Team Awesome Oscillators]]<br>
+Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]

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