Calgary

From 2007.igem.org

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<td bgcolor="#006633"><p align="center" style="font-size:20px; color:#FFFFFF;"> E. coLisa</p></td>
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<td bgcolor="#006633"><p align="center" style="font-size:20px; color:#FFFFFF;"> <em>E. co</em>Lisa</p></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/choosing_our_project" title="choosing our project">Choosing Our Project</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/choosing_our_project" title="choosing our project">Project</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/design" title="Designing Our Project">Designing Our Project</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/design" title="Designing Our Project">Designing: Wet Lab</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/testing" title="Testing Parts and Primers">Testing Parts And Primers</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/testing" title="Testing Parts and Primers">Testing</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/constructing_wetlab" title="Construction: Wetlab Component">Construction Our Project: The Wetlab</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/constructing_wetlab" title="Construction: Wetlab Component">Constructing Our Project: The Wetlab</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/constructing_printer" title="Construction: Printer and Software">Constructing Our Project: Printer and Software</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/constructing_printer" title="Construction: Printer and Software">Design: Printer</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/final_result" title="Final Result">Final Result of E. coLisa</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/final_result" title="Final Result">Final Result of <em>E. co</em>Lisa</a></td>
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<td><a class="sideLinks" href="" title="Project Description">Project Description</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/evoGEM_introduction" title="Project Description">Introduction to evoGEM </a></td>
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<td><a class="sideLinks" href="" title="Results">Results</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/evoGEM_projectDesign" title="Results">Project Design</a></td>
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<td><a class="sideLinks" href="" title="Simulations">Simulations</a></td>
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<td><a class="sideLinks" href="https://2007.igem.org/Calgary/evoGEM_results" title="Simulations">Results</a></td>
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Revision as of 03:57, 27 October 2007

Simplified Project Plan Our Team See Our Sponsors Fun Stuff

E. coLisa
Project
Designing: Wet Lab
Testing
Constructing Our Project: The Wetlab
Design: Printer
Protocols
Final Result of E. coLisa

evoGEM
Introduction to evoGEM
Project Design
Results
Welcome to the University of Calgary Wiki for the 2007 iGEM Competition

This site presents detailed information about both of our projects. We have two projects that we are entering in this compeition. The first Eco. Lisa is our biological entry, the second Evo Gem is our computational project. Full descriptions of each project can be found by using the links to your left. We are updating this page with new information for the public on an ongoing basis!

E. coLisa What It Is and Where We Are...

The primary UofC iGEM project for 2007 is to design and build a biomechanical printer; composed of a two dimensional plotter equipped with a red laser, software to translate computer images into instructions for the plotter, and E. coli cells engineered to respond to the laser light. Bacteria are spread in a solid lawn on the plate, or mixed in the media before pouring the plate. The response triggered by this biological circuit is to produce beta agarase, an enzyme which degrades the agar polymer that the cells rest on.

By varying the amount of light exposure each point on the plate receives, we can control the amount of agarase expression, and so the height of the agar gel, at each location. The result is bacterial lithography. In particular, the fine resolution achievable by laser control could allow resolution as high as 10 megapixels per inch, precise enough to target individual cells.

At the moment we have our logic circut builit and placed in a biobrick plasmid. Agarase is still in the process of being isolated and our light sensing component is still understruction

 evoGEM What It Is and Where We Are...

At this point our evoGEM project is ready to show. It will accept a variety of parametres and automatically begin to generate potential circuts

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