Chiba/Making Marimo
From 2007.igem.org
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==Parts Construction== | ==Parts Construction== | ||
Because we found that FliC code includes restriction enzyme(EcoRI,SpeI,PstI) which is used in Biobrick, we divided into plasmid of FliC and one of signal, and aimed the double transformation. | Because we found that FliC code includes restriction enzyme(EcoRI,SpeI,PstI) which is used in Biobrick, we divided into plasmid of FliC and one of signal, and aimed the double transformation. | ||
| - | ===[[Chiba/Flagella/FliC-his_generator|FliC-His generator]]=== | + | ===[[Chiba/Flagella/FliC-his_generator|Moving FliC-His generator]]=== |
====Experiment==== | ====Experiment==== | ||
[[Image:FliC-His ligation.jpg|frame|Fig2.Ligation Strategy]] | [[Image:FliC-His ligation.jpg|frame|Fig2.Ligation Strategy]] | ||
Revision as of 04:25, 27 October 2007
|
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal |
Making Marimos
Fig1. Scheme of final gene cuircuit for Bacteria Marimo.
Parts Construction
Because we found that FliC code includes restriction enzyme(EcoRI,SpeI,PstI) which is used in Biobrick, we divided into plasmid of FliC and one of signal, and aimed the double transformation.
Moving FliC-His generator
Experiment
Fig2.Ligation Strategy
- We regulate the expression of His-tagged FliC by lux promoter. Namely, if LuxR is expressed, bacteria can express FliC via Quorum Seinsing.
- Because the Quorum Sensing device is on ColE1-type vector, we need to export the FliC unit into the plasmids with compatible origins such as p15A.
Results
First attempt to import FliC-His generator into p15A vector (see Fig).
Communication units are on ColE1 type plasmids.
To make this stickly FliC construct compatible with communication circuits, this is an absolute necessity.....
