Tokyo Tech

From 2007.igem.org

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(<span style="color:#ff33cc;">Wet assay </span>)
(4. Works)
 
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[[Image:Tokyotech4.JPG|900px]]
[[Image:Tokyotech4.JPG|900px]]
__NOTOC__
__NOTOC__
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==[[Tokyo_Tech|Abstract]]  [[Tokyo/Model|Concept & Model]]  [[Tokyo/Requirements |Requirements]]  [[Tokyo/Genetic circuit|Genetic_circuit]]  [[Tokyo/Works|Works]]  [[Tokyo/About our team|About_our_team]]==
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==[[Tokyo_Tech|Abstract]]&nbsp;&nbsp;&nbsp;[[Tokyo/Model|Concept & Model]]&nbsp;&nbsp;&nbsp;[[Tokyo/Requirements |Requirements]]&nbsp;&nbsp;&nbsp;[[Tokyo/Genetic circuit|Genetic_circuit]]&nbsp;&nbsp;&nbsp;[[Tokyo/Works|Works]]&nbsp;&nbsp;&nbsp;[[Tokyo/About our team|About_our_team]]==
<h1> ''' Abstract''' </h1>
<h1> ''' Abstract''' </h1>
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|width="500px" valign="top" |  
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==1. [[Tokyo/Model|Concept & Model]]==
==1. [[Tokyo/Model|Concept & Model]]==
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'''The goal of our project is to make a bacterial society that follows Pareto's principle as an [[Tokyo/Concepts|ant society]] does. ([[Tokyo/Model|See more.]])''' [[Image:TOP.JPG|470px]]
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'''The goal of our project is to make a bacterial society that follows Pareto's principle as an [[Tokyo/Concepts|ant society]] does.
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このfigの下に、赤の個体の絵:worker、個体の絵:idelerと記す。
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'''On the other word, we try to construct a bacterial system which takes "balanced differentiation".([[Tokyo/Model|See more.]])'''
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<br>'''1. Coexistence ⇒ 2. Worker Removal ⇒ 3.Coexistence again'''
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<br>[[Image:Defferentiation4.JPG|450px]]
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<br>'''1. Differentiation => 2. Worker Removal '''
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<br> '''3. Dedifferentiation => 4.Redifferentiation'''
==2. [[Tokyo/Requirements |Requirements]]==
==2. [[Tokyo/Requirements |Requirements]]==
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'''Bistability and cell-cell communication are necessary to realize our model of "stable coexistence". ([[Tokyo/Requirements|See more.]])'''
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'''Bistability and cell-cell communication are necessary to realize our model of "Balanced differentiation". ([[Tokyo/Requirements|See more.]])'''
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<br>[[Image:Bistability.JPG|180px]][[Image:Cell-Cell.JPG|300px]]
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<br><br><br>
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'''1.Differentiation generated by mutual-inhibition circuit.'''
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<!-- '''1.Bistability generated by one genetic circuit.''' -->
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<br>'''2.Cell-cell communication .'''
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<br>[[Image:Cellcell.JPG|450px]]
==3. [[Tokyo/Genetic circuit|Genetic circuit]]==
==3. [[Tokyo/Genetic circuit|Genetic circuit]]==
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'''The genetic circuit in this project consisted of [[Tokyo/Genetic circuit/the new parts|the new parts]] as well as BioBrick parts. ([[Tokyo/Genetic circuit|See more.]])'''
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'''The genetic circuit in this project consisted of [[Tokyo/Genetic circuit/the new parts|the new parts]] as well as BioBrick parts. (See more for [[Tokyo/Genetic circuit|the genetic circuit]] and [[Tokyo/Genetic circuit/the new parts|the new parts]])'''
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<!--(木賀)biobrickがdeliverされたのは当然なのでこの単語を消去-->
<!--(木賀)biobrickがdeliverされたのは当然なのでこの単語を消去-->
<!--ここで言いたいことはgenetic circuitの大きさと自分達が作った新しいパーツを使っているということ-->
<!--ここで言いたいことはgenetic circuitの大きさと自分達が作った新しいパーツを使っているということ-->
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<br>[[Image:Geneticcurcuit.JPG|290px|left]]
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[[Image:This is the new part.JPG|150px|none]]
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<br><br>'''The new parts of this year are [[Tokyo/Genetic circuit/the new parts|here]]!!'''
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<br>[[Image:Tokyo_genetic circuit_s.jpg|280px|left]]
 
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[[Image:hybridgraph3.JPG|thumb|150px|'''Assay of our brand-new promoter part'''|none]]
 
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==4. [[Tokyo/Works|Works]]==
==4. [[Tokyo/Works|Works]]==
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'''Our model has been sophisticated though successive combinations of wet and dry approaches. ([[Tokyo/Works|See more.]])'''
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'''Our model has been sophisticated through successive combinations of wet and dry approaches. ([[Tokyo/Works|See more.]])'''
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'''<span style="color:#ff33cc;">Wet assay </span>'''
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<br>[[Image:hill AHL.JPG|200px]][[Image:hill IPTG.JPG|200px]]
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====<span style="color:#ff33cc;">Wet assay </span>====
 
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<br>[[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]]
 
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<br>Figの縦軸 fluorescence(AU) 横軸 concentration(単位)を見える大きさの文字で書く
 
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====<span style="color:#0066ff;">Dry simulation </span>====
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'''<span style="color:#0066ff;">Dry simulation </span>'''
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Using the data from wet experiments
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'''<br>Using the data from wet experiments,the guideline for the design of the model was established'''
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
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<br>[[Image:Defferentiation result.JPG|450px]]
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<!--<br>[[Image:Tokyo_simulation_s4.jpg|300px]]-->
== 5. [[Tokyo/About our team |About our team]] ==
== 5. [[Tokyo/About our team |About our team]] ==
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'''Instructors'''
'''Instructors'''
<br>[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
<br>[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
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[[Tokyo/yarubeki koto|やるべきこと]]
 
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<br>
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<br>
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<br>
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== ==
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'''[[Tokyo/Reference|Reference]]'''

Latest revision as of 04:30, 27 October 2007

Tokyotech4.JPG


Abstract   Concept & Model   Requirements   Genetic_circuit   Works   About_our_team

Abstract

1. Concept & Model

The goal of our project is to make a bacterial society that follows Pareto's principle as an ant society does. On the other word, we try to construct a bacterial system which takes "balanced differentiation".(See more.)
Defferentiation4.JPG
1. Differentiation => 2. Worker Removal
 3. Dedifferentiation => 4.Redifferentiation

2. Requirements

Bistability and cell-cell communication are necessary to realize our model of "Balanced differentiation". (See more.)


1.Differentiation generated by mutual-inhibition circuit.
2.Cell-cell communication .
Cellcell.JPG

3. Genetic circuit

The genetic circuit in this project consisted of the new parts as well as BioBrick parts. (See more.)


Geneticcurcuit.JPG
This is the new part.JPG



The new parts of this year are here!!


4. Works

Our model has been sophisticated through successive combinations of wet and dry approaches. (See more.)

Wet assay
Hill AHL.JPGHill IPTG.JPG


Dry simulation
Using the data from wet experiments,the guideline for the design of the model was established

Defferentiation result.JPG

5. About our team

Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Shotaro Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]




Reference