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	== Background ==		== Background ==
		+	{| border="0" cellspacing="8px" cellpadding="15" width="80%"
		+	|-
		+	|[[Berkeley_LBL|Home]]
		+	|[[Berkeley_LBL/Project|Project Description]]
		+	|[[Berkeley_LBL/Methods|Methods]]
		+	|[[Berkeley_LBL/Laina Notebook|Laina's Notebook]]
		+	|[[Berkeley_LBL/Results|Results and Discussion]]
		+	|[[Berkeley_LBL/Resources|Resources]]
		+	|}
		+	[[Image:grouplab.jpg]] No idea. ask Connie
-	[[Image:lainalab.jpg]] Pretending                  [[Image:grouplab.jpg]] No idea. ask Connie	+	My name is Laina.
-		+
-	My name is Laina Magaya,  1st year Graduate Student,	+	This year I am on the UCB-LBNL IGEM team.
-	Chemistry Prairie View.	+
-		+
-	This year I am on the UCB-LBNL IGEM team.	+
-	My task this summer is to isolate and overexpress bacteriochlorophyll	+
-	biosynthetic genes of the H. mobilis photosynthetic gene cluster. This is achieved by using  upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX.	+
	== Project ==		== Project ==
-	View My Presentation
-	Presentaion	+	'''Introduction'''
		+	My task this summer is to isolate and overexpress protochlorophyalide reductase genes (bch-L,-N and -M) and bacteriochlorophyll biosynthetic genes (bch M, N, E, B,and I)of the H. mobilis photosynthetic gene cluster. This is achieved by using  upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX.
-	----	+	A genomic library was constructed by partial digestion with EcoRI/BamHI and BgII/XhoI followed by ligation of the DA fragments into EcoRI/XhoI  abd BgII/XhoI site of the cosmid vector pET29bEBBX. Oligonucleotide primers were synthesized by Intergrated DNA technologies (IDT),Inc, Coraville, IA. Nucleotide sequence analysis was analysed using Lasergene Megasoftware. Database search was done using Basic Local Alignment Search Tool (BLAST).
		+	Sequence Compilation
		+	Sequence Alignment
-	[[Image:IGEMPresentaion.jpg]]	+	{| border = "1"
		+	|-
		+	|[[Image:bacter1.jpg]]
		+	|
		+	'''bacteriochlorophyll Biosynthesis Pathway'''
-	== Summer Project Diary ==	+	The presence  photosynthetic gene cluster involved in the steps of the bacteriochlorophyll/chlorophyll biosynthesis pathway between Mg-chelation and formation of chlorophyllide (bchI, bchD, bchH, bchJ, bchM,bchE,bchL,bchN and bchB) have been found by inspection of the ORFs (Xion ''et al'').
-	June	+
		+	|}
-	July	+	== Presentation ==
-		+	[[Image:IGEMPresentaion.jpg]]
-	August	+

---

Latest revision as of 06:14, 27 October 2007

Background

Home

Project Description

Methods

Laina's Notebook

Results and Discussion

Resources

            No idea. ask Connie

My name is Laina.

This year I am on the UCB-LBNL IGEM team.

Project

Introduction My task this summer is to isolate and overexpress protochlorophyalide reductase genes (bch-L,-N and -M) and bacteriochlorophyll biosynthetic genes (bch M, N, E, B,and I)of the H. mobilis photosynthetic gene cluster. This is achieved by using upstream ribosome binding sites, downstream from the T7 promoter site, for expression optimization in a high copy number plasmid pET-29a modified to pET29-b-EBBX.

A genomic library was constructed by partial digestion with EcoRI/BamHI and BgII/XhoI followed by ligation of the DA fragments into EcoRI/XhoI abd BgII/XhoI site of the cosmid vector pET29bEBBX. Oligonucleotide primers were synthesized by Intergrated DNA technologies (IDT),Inc, Coraville, IA. Nucleotide sequence analysis was analysed using Lasergene Megasoftware. Database search was done using Basic Local Alignment Search Tool (BLAST). Sequence Compilation Sequence Alignment

File:Bacter1.jpg

bacteriochlorophyll Biosynthesis Pathway The presence photosynthetic gene cluster involved in the steps of the bacteriochlorophyll/chlorophyll biosynthesis pathway between Mg-chelation and formation of chlorophyllide (bchI, bchD, bchH, bchJ, bchM,bchE,bchL,bchN and bchB) have been found by inspection of the ORFs (Xion et al).

Presentation