NYMU Taipei/Construct
From 2007.igem.org
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<li>May be checked with: BtgZI</li> | <li>May be checked with: BtgZI</li> | ||
<li>8/27, 2007</li> | <li>8/27, 2007</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <hr /> | ||
+ | <h2>OmpRBS assay construct: pLacIQ + OmpRBS + GFP</h2> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>[[Image:PLacIQ-OmpRBS-GFP (plasmid).jpg|300px]]</td> | ||
+ | <td> | ||
+ | <ul> | ||
+ | <li>our first construct for OmpRBS blocking assay under glucose stimulus</li> | ||
+ | <li>Insert size: 1004bp (XbaI to SpeI)</li> | ||
+ | <li>Total vector size: 5,425bp</li> | ||
+ | <li>V-I: 4,421bp</li> | ||
+ | <li>May be checked with: BtgZI</li> | ||
+ | <li>10/24, 2007</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <hr /> | ||
+ | <h2>OmpRBS assay construct: pLacIQ + OmpRBS</h2> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>[[Image:PLacIQ-OmpRBS (plasmid).jpg|300px]]</td> | ||
+ | <td> | ||
+ | <ul> | ||
+ | <li>our first construct for OmpRBS blocking assay under glucose stimulus</li> | ||
+ | <li>Insert size: 120bp (XbaI to SpeI)</li> | ||
+ | <li>Total vector size: 4,541bp</li> | ||
+ | <li>V-I: 4,421bp</li> | ||
+ | <li>May be checked with:</li> | ||
+ | <li>12/13, 2007</li> | ||
</ul> | </ul> | ||
</td> | </td> | ||
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<h3>Phase 4 of system 1: pOmpC + TATA_INSA + TATB_INSB <font color="#ff0000">+ </font>CinR+HSL+D-term</h3> | <h3>Phase 4 of system 1: pOmpC + TATA_INSA + TATB_INSB <font color="#ff0000">+ </font>CinR+HSL+D-term</h3> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>[[Image:System I (plasmid).jpg|300px]]</td> | ||
+ | <td> | ||
+ | <ul> | ||
+ | <li>Insert size: 2,139bp</li> | ||
+ | <li>Total vector size: 4,292bp</li> | ||
+ | <li>V-I: 2,153bp</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<hr /> | <hr /> | ||
<h2>System 2: pCinRHSL+ OmpRBS + (TATD+IDE) + D-term</h2> | <h2>System 2: pCinRHSL+ OmpRBS + (TATD+IDE) + D-term</h2> | ||
<h3>Phase 1 of system 2: TATD <font color="#ff0000">+</font> IDE = TATD_IDE</h3> | <h3>Phase 1 of system 2: TATD <font color="#ff0000">+</font> IDE = TATD_IDE</h3> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>[[Image:TATD IDE (insert).jpg|300px]]</td> | ||
+ | <td> | ||
+ | <ul> | ||
+ | <li>Insert size: 3,190bp</li> | ||
+ | <li>PCR product size: 3,203bp</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<h3>Phase 1 of system 2: pCinRHSL <font color="#ff0000">+</font> OmpRBS</h3> | <h3>Phase 1 of system 2: pCinRHSL <font color="#ff0000">+</font> OmpRBS</h3> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>[[Image:PCinRHSL-OmpRBS (plasmid).jpg|300px]]</td> | ||
+ | <td> | ||
+ | <ul> | ||
+ | <li>Insert size: 308bp</li> | ||
+ | <li>Total vector size: 2,461bp</li> | ||
+ | <li>V-I: 2,153bp</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<h3>Phase 2 of system 2: pCinRHSL + OmpRBS <font color="#ff0000">+</font> TATD_IDE</h3> | <h3>Phase 2 of system 2: pCinRHSL + OmpRBS <font color="#ff0000">+</font> TATD_IDE</h3> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>[[Image:PCinRHSL-OmpRBS-TATD IDE (plasmid).jpg|300px]]</td> | ||
+ | <td> | ||
+ | <ul> | ||
+ | <li>Insert size: 3,488bp</li> | ||
+ | <li>Total vector size: 5,641bp</li> | ||
+ | <li>V-I: 2,153bp</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | |||
<h3>Phase 3 of system 2: pCinRHSL + OmpRBS + TATD_IDE <font color="#ff0000">+</font> D-term</h3> | <h3>Phase 3 of system 2: pCinRHSL + OmpRBS + TATD_IDE <font color="#ff0000">+</font> D-term</h3> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <td>[[Image:System 2 (plasmid).jpg|300px]]</td> | ||
+ | <td> | ||
+ | <ul> | ||
+ | <li>Insert size: 3,589bp</li> | ||
+ | <li>Total vector size: 5,742bp</li> | ||
+ | <li>V-I: 2,153bp</li> | ||
+ | </ul> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<hr /> | <hr /> | ||
+ | |||
<h2>Basic Constructs</h2> | <h2>Basic Constructs</h2> | ||
<h3>pOmpC in pSB1A2</h3> | <h3>pOmpC in pSB1A2</h3> |
Latest revision as of 04:00, 13 December 2007
Assembly Ordering and Progress
system 1
System 1: glucose-sensing insulin generator (5 task left: 2 PCR and 3 biobrick standard assembly) |
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pOmpC | RBS + TAT |
INSA | RBS + TAT |
INSB | RBS | CinR | RBS | HSL | D-term | duration | members | assembly | status |
RBS + CinR | RBS + HSL | 8/30-8/31 | 翔 | 1st assembly | 9/01, 07 |
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CinR + HSL (RBS+) | 9/1-9/4 | 宥,君,威 | 2nd assembly | 9/02, 07 |
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CinR+HSL(RBS+) + D-term | 9/16-9/27 | 宥,君,翔,pett | 3rd assembly | 9/27 07 |
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RBS + TATA+ INSA (PCR) | RBS + TATB+ INSB (PCR) | 9/28-9/30 |
銘, pett |
basic part (PCR) | 9/30, 07 |
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pOmpC + TATA_INSA (failed) | TATB_INSB + CinR+HSL+D-term (wrong assembly) |
-10/5 | pett | 4th assembly | 10/5,7,8 07 |
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pOmpC + TATA_INSA | -10/11 | pett | 4th assembly | ||||||||||
pOmpC + TATA_INS_A + TATB_INS_B |
-10/23 | 4th assembly | |||||||||||
pOmpC + TATA_INS_A + TATB_INS_B + CinR+HSL+D-term | Final |
system 2
System 2: insulin-sensing insulin destoryer (5 tasks left: 2 PCR and 3 biobrick standard assembly) |
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pCinRHSL | OmpRBS | RBS + TATD |
IDE | D-term | duration | members | assembly | status |
OmpRBS (PCR) | RBS + TATD+ IDE (PCR) |
-102 |
銘, 茹, pett |
basic part (PCR) | 10/2 07 |
|||
pCinRHSL + OmpRBS | -10/5 | pett | 1st assembly | 10/5 07 |
||||
pCinRHSL + OmpRBS + TATD_IDE (RBS+) | -10/11 | pett | 2nd assembly | |||||
pCinRHSL+OmpRBS+TATD_IDE + D-term | Final |
Promoter assay construct: pOmpC+(RBS+EYFP)
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OmpRBS assay construct: pLacIQ + OmpRBS + GFP
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OmpRBS assay construct: pLacIQ + OmpRBS
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System 1: pOmpC + (TATA+INSA) + (TATB+INSB) + (RBS+CinR) + (RBS+HSL) + D-term
Phase 1 of system 1: RBS + CinR
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Phase 1 of system 1: RBS + HSL
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Phase 1 of system 1: TATA + INSA = TATA_INSA
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Phase 1 of system 1: TATB + INSB = TATB_INSB
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Phase 2 of system 1: pOmpC + TATA_INSA
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Phase 2 of system 1: (RBS+CinR) + (RBS+HSL) = CinR+HSL
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Phase 3 of system 1: pOmpC + TATA_INSA + TATB_INSB
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Phase 3 of system 1: CinR+HSL + D-term = CinR+HSL+D-term
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Phase 4 of system 1: pOmpC + TATA_INSA + TATB_INSB + CinR+HSL+D-term
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System 2: pCinRHSL+ OmpRBS + (TATD+IDE) + D-term
Phase 1 of system 2: TATD + IDE = TATD_IDE
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Phase 1 of system 2: pCinRHSL + OmpRBS
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Phase 2 of system 2: pCinRHSL + OmpRBS + TATD_IDE
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Phase 3 of system 2: pCinRHSL + OmpRBS + TATD_IDE + D-term
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Basic Constructs
pOmpC in pSB1A2
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pCinRHSL in pSB1A2
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RBS in pSB1A2
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D-term in pSB1AK3
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