NYMU Taipei/Part List

From 2007.igem.org

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<p><font size="4">
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[[NYMU_Taipei/ProjectDescription| Project Description]] /
 +
[[NYMU_Taipei/Lab_Notes| Lab Notes]]  /
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[[NYMU_Taipei/Part_List| Part List]]  /
 +
[[NYMU_Taipei/Primer_List| Primer List]]
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</font><br /><hr></p>
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==Part List==
{| style="color:green;background-color:#ffffcc;" cellpadding="20" cellspacing="0" border="1"
{| style="color:green;background-color:#ffffcc;" cellpadding="20" cellspacing="0" border="1"
!Series
!Series
Line 5: Line 13:
!Description
!Description
!part length
!part length
-
!plasmid (antibotics)
+
!vector (antibotics)
 +
!Check
|-
|-
|[http://partsregistry.org/Part:BBa_R0082 BBa_R0082]
|[http://partsregistry.org/Part:BBa_R0082 BBa_R0082]
Line 13: Line 22:
|108
|108
|pSB1A2
|pSB1A2
 +
|?
|-
|-
|[http://partsregistry.org/Part:BBa_C0082 BBa_C0082]
|[http://partsregistry.org/Part:BBa_C0082 BBa_C0082]
Line 27: Line 37:
|12
|12
|pSB1A2
|pSB1A2
 +
|?
|-
|-
|AU43-C7
|AU43-C7
Line 52: Line 63:
|D-term
|D-term
|1,2,3
|1,2,3
-
|Double translation terminator
+
|Double transcription terminator
|95
|95
|pSB1AK3
|pSB1AK3
 +
|OK
|-
|-
|[http://partsregistry.org/Part:BBa_I14032 BBa_I14032]
|[http://partsregistry.org/Part:BBa_I14032 BBa_I14032]
Line 67: Line 79:
|2
|2
|OmpR binding sites
|OmpR binding sites
 +
|65
|
|
-
|
+
|?
|-
|-
|AV88-C6
|AV88-C6
Line 94: Line 107:
|B0032.ECFP
|B0032.ECFP
|16D, plate 1
|16D, plate 1
-
|his part accepts POPS to produce ECFP under the medium RBS B0032
+
|This part accepts POPS to produce ECFP under the medium RBS B0032
|742
|742
|pSB1A2
|pSB1A2
 +
|OK
|-
|-
|[http://partsregistry.org/Part:BBa_I15017 BBa_I15017]
|[http://partsregistry.org/Part:BBa_I15017 BBa_I15017]
Line 104: Line 118:
|742
|742
|pSB1A2
|pSB1A2
 +
|OK
 +
|-
 +
|[http://partsregistry.org/Part:BBa_E0840 BBa_E0840]
 +
|B0030(RBS).E0040(GFP).B0015(D-term)
 +
|16E, plate 1
 +
|gfp tri-part; strong rbs
 +
|878
 +
|pSB1A2
 +
|OK
|-
|-
|[http://partsregistry.org/Part:BBa_R0077 BBa_R0077]
|[http://partsregistry.org/Part:BBa_R0077 BBa_R0077]
|pCinRHSL, RBS+
|pCinRHSL, RBS+
|17L, Plate 1
|17L, Plate 1
-
|
+
|CinR-HSL complex binds to this promoter and activates transcription. This part contains RBS.
|231
|231
|pSB1A2
|pSB1A2
 +
|OK
|-
|-
|-
|-
Line 116: Line 140:
|pCinRHSL
|pCinRHSL
|15D, Plate 1
|15D, Plate 1
-
|
+
|CinR-HSL complex binds to this promoter and activates transcription. This part DOES NOT contain RBS.
|225
|225
|pSB1A2
|pSB1A2
-
|-
+
|?
|-
|-
|[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
|[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
|CinR
|CinR
|7G, Plate 2  
|7G, Plate 2  
-
|
+
|cinR activator
|762  
|762  
|pSB2K3
|pSB2K3
 +
|OK
|-
|-
|[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
|[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
|HSL
|HSL
|7E, Plate 2
|7E, Plate 2
-
|
+
|autoinducer
|702
|702
|pSB2K3
|pSB2K3
 +
|OK
|-
|-
|}
|}
-
* antibiotics keys
+
 
-
** A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
+
==antibiotics keys==
-
** K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
+
* A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
-
** C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
+
** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
 +
** stock (100 mg/mL = 100 ug/uL) ampicillin
 +
** liquid culture (50 ug/mL = 250 ug/5mL)
 +
** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicillin
 +
* K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
 +
* C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
 +
** 35 mg/mL
 +
** 20 mg/mL in Ethanol
 +
** we prepared 100mg in 5 mL and stored in -20 C

Latest revision as of 09:08, 24 January 2008

Project Description / Lab Notes / Part List / Primer List

Part List

Series alias Location Description part length vector (antibotics) Check
[http://partsregistry.org/Part:BBa_R0082 BBa_R0082] pOmpC 1 Positively regulated, OmpR-controlled promoter 108 pSB1A2 ?
[http://partsregistry.org/Part:BBa_C0082 BBa_C0082] tar-envZ 1 Receptor, tar-envZ 1491 pSB2K3
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034] RBS 1,2,3 The Elowitz RBS (Ribosome binding site) is the definition of efficiency 1.0 12 pSB1A2 ?
AU43-C7 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=13528923 INS] human insulin 495 C
INS_A 1 alpha chain of human insulin 63
INS_B 1 beta chain of human insulin 90
[http://partsregistry.org/Part:BBa_B0014 BBa_B0014] D-term 1,2,3 Double transcription terminator 95 pSB1AK3 OK
[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] pLacIQ 2 Constitutive Promoter, High Transcription 37 pSB2K3
OmpRBS 2 OmpR binding sites 65 ?
AV88-C6 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27371195 IDE] 2 insulin degradation enzyme of mouse 3445 A
AU61-F6 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=33879043 IGFBP3] 2 Insulin-like growth factor binding protein 3 of human 2488 C
AU50-C7 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=38114635 IGFBP7] 2 Insulin-like growth factor binding protein 7 of human 1155 C
[http://partsregistry.org/Part:BBa_I15016 BBa_I15016] B0032.ECFP 16D, plate 1 This part accepts POPS to produce ECFP under the medium RBS B0032 742 pSB1A2 OK
[http://partsregistry.org/Part:BBa_I15017 BBa_I15017] B0032.EYFP 16F, plate 1 This part accepts POPS to generate EYFP under the medium strength RBS B0032 742 pSB1A2 OK
[http://partsregistry.org/Part:BBa_E0840 BBa_E0840] B0030(RBS).E0040(GFP).B0015(D-term) 16E, plate 1 gfp tri-part; strong rbs 878 pSB1A2 OK
[http://partsregistry.org/Part:BBa_R0077 BBa_R0077] pCinRHSL, RBS+ 17L, Plate 1 CinR-HSL complex binds to this promoter and activates transcription. This part contains RBS. 231 pSB1A2 OK
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078] pCinRHSL 15D, Plate 1 CinR-HSL complex binds to this promoter and activates transcription. This part DOES NOT contain RBS. 225 pSB1A2 ?
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077] CinR 7G, Plate 2 cinR activator 762 pSB2K3 OK
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076] HSL 7E, Plate 2 autoinducer 702 pSB2K3 OK

antibiotics keys

  • A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
    • refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
    • stock (100 mg/mL = 100 ug/uL) ampicillin
    • liquid culture (50 ug/mL = 250 ug/5mL)
    • 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicillin
  • K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
  • C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
    • 35 mg/mL
    • 20 mg/mL in Ethanol
    • we prepared 100mg in 5 mL and stored in -20 C
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