USTC

From 2007.igem.org

(Difference between revisions)
m (→Our Project:<BR>Extensible Logic Circuit in Bacteria)
m
 
(32 intermediate revisions not shown)
Line 18: Line 18:
=== Our Project:<BR>Extensible Logic Circuit in Bacteria ===
=== Our Project:<BR>Extensible Logic Circuit in Bacteria ===
-
Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.  
+
Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit <i>in vivo</i> with several levels of gates.
-
Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing cis-acting elements, favored for its small scale and potential to implement complicated logic computation in vivo, can be systematically built up, and act as a gate. Meanwhile, artificial repressors with highly-specified DNA-recognition regions are able to transmit signals without mutual interference, just as copper wires. In this way, a circuit can be constructed regardless of the number of logic gates and the manner of connections between the wires.  
+
Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation <i>in vivo</i>, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.
A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?
A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?
 +
<font color="#AAAAAA">''For detailed description, please click each hyperlink below.''</font>
|width=330px style="padding: 5px" |
|width=330px style="padding: 5px" |
Line 29: Line 30:
[[Image:DSCN1243.png|330px]]
[[Image:DSCN1243.png|330px]]
-
''From left to right''<BR>
+
<font color="#AAAAAA">''From left to right''</font><BR>
-
''Back row:'' [[USTC/ZhaoYun|Zhao Yun]], [[USTC/LiuZiqing|Liu ZQ]], [[USTC/MaXiaoyu|Ma XY]]<BR>
+
<font color="#AAAAAA">''Back row:''</font> [[USTC/ZhaoYun|Zhao Yun]], [[USTC/LiuZiqing|Liu ZQ]], [[USTC/MaXiaoyu|Ma XY]]<BR>
-
''Front row:'' [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su XF]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
+
<font color="#AAAAAA">''Front row:''</font> [[USTC/MaRui|Ma Rui]], [[USTC/SuXiaofeng|Su XF]], [[USTC/DingBo|Ding Bo]], [[USTC/ZhanJian|Zhan Jian]]
<BR clear="both">
<BR clear="both">
Line 53: Line 54:
<h3>Project</h3>
<h3>Project</h3>
-
<font color="#AAAAAA">''For detailed description, please click each hyperlink below.''</font>
 
-
 
-
 
Line 88: Line 86:
<h3>Members</h3>
<h3>Members</h3>
-
 
<center>
<center>
Line 113: Line 110:
</center>
</center>
 +
|width=240px style="padding: 5px; background-color: #FFFF99" |
 +
<h3>Snapshot</h3>
-
----
+
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC '''<font color="#bb0000">247</font> Part Sequences''']
-
''Department of Systems Biology''
+
-
''Department of Molecular Biology<br>and Cellular Biology''
+
'''<font color="#bb0000">123</font> Parts Submitted'''
-
''Department of Chemical Physics''
 
 +
'''77''' Synthesized Promoters
-
|width=240px style="padding: 5px; background-color: #FFFF99" |
+
'''11''' Novel Artificial Repressors
-
<h3>Statistics</h3>
 
-
In more than 4 months, our
 
-
'''Parts designed'''<br>
+
'''~ 350''' New Strains
-
= <font color="#bb0000">'''229'''</font> parts ...[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Go to see them]<br>
+
-
'''Parts submitted'''<br>
+
-
= <font color="#bb0000">'''123'''</font> parts
+
 +
'''~ 130''' DNA Strands Sequenced
-
'''Oligo-synthesis'''<br>
+
'''> 5000''' Colonies Screened
-
~ 120 fragments<br>
+
-
'''PCR synthesizing and detection'''<br>
+
-
~ 800 times<br>
+
-
'''Restriction digestion'''<br>
+
-
> 600 times<br>
+
-
'''Transformation'''<br>
+
-
~ 300 times
+
 +
'''~ 400''' Quantitative Assays
-
'''DNA sequencing'''<br>
 
-
~ 130 times<br>
 
-
'''Fluorescence detection'''<br>
 
-
> 100 times<br>
 
-
'''Fluorescent Density measuring'''<br>
 
-
> 200 times<br>
 
-
'''ONPG assay'''<br>
 
-
~ 70 times<br>
 
-
<!--- <font color="#bb0000">'''Synthesis'''</font> --->
 
-
[[USTC/Events|'''Events''']]
+
<br>
 +
<br>
 +
<br>
 +
[[USTC/Events|'''Previous Events''']]
|-valign="top"
|-valign="top"

Latest revision as of 10:51, 8 March 2011


USTC Logo.png

Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.

A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?

For detailed description, please click each hyperlink below.

DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Project


Introduction


Core Logic Components:

Logic-Gate Promoters


Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico


Peripheral Devices:

Inputs and Outputs


Extensible System:

A Demonstration

Further More


Members


4 Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu


3 Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun


3 Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Snapshot


[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC 247 Part Sequences]

123 Parts Submitted


77 Synthesized Promoters

11 Novel Artificial Repressors


~ 350 New Strains

~ 130 DNA Strands Sequenced

> 5000 Colonies Screened

~ 400 Quantitative Assays






Previous Events

Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]

Retrieved from "http://2007.igem.org/wiki/index.php/USTC"