June 18
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* pLac cfp Inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (evening). | * pLac cfp Inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (evening). | ||
| - | * The new constructs viz. | + | * The new constructs viz. j22261,j22271,j22281,j22301 were transformed. |
* pLac yfp induced with various conc of IPTG (0,1,10,50,100,1000 uM). | * pLac yfp induced with various conc of IPTG (0,1,10,50,100,1000 uM). | ||
* pLac cfp induced with various conc of IPTG (0,1,10,50,100,1000 uM). | * pLac cfp induced with various conc of IPTG (0,1,10,50,100,1000 uM). | ||
| + | |||
| + | * For FACS recalibration another set of pLac cfp was induced with IPTG levels of 0,10,100,1000 uM. | ||
Latest revision as of 08:36, 28 June 2007
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Experiments
- pLac yfp and pLac luxI cfp could not be imaged because the OD was found to be greater than 1.
- pLac yfp inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (morning).
- pLac cfp Inoculated in 3ml LB+3ul amp and K12Z1 in 3ml LB+3ul spec (evening).
- The new constructs viz. j22261,j22271,j22281,j22301 were transformed.
- pLac yfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
- pLac cfp induced with various conc of IPTG (0,1,10,50,100,1000 uM).
- For FACS recalibration another set of pLac cfp was induced with IPTG levels of 0,10,100,1000 uM.
