Template:BerkiGEM2007 HannahConstructionFiles

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
-
 
[[BerkiGEM2007-HannahConstructionFile1 | Construction of I716316]]<br>
[[BerkiGEM2007-HannahConstructionFile1 | Construction of I716316]]<br>
-
 
-
 
-
 
-
PCR HNX-F and HNX-R on H-Nox  (567bp, BglII/XhoI)
 
-
Sub into pBca1144              ( BglII/XhoI, 2054+910  L)
 
-
Product is pBca1144-H-NOX
 
-
----------------------------------------------------------
 
-
HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG
 
-
 
-
HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA
 

Revision as of 21:33, 28 June 2007

Construction of I716316

Retrieved from "http://2007.igem.org/wiki/index.php/Template:BerkiGEM2007_HannahConstructionFiles"