From 2007.igem.org

Revision as of 08:58, 29 June 2007 (view source) Vgautam (Talk | contribs) ← Older edit		Revision as of 11:36, 29 June 2007 (view source) Vgautam (Talk | contribs) (→Experiments) Newer edit →
Line 3:		Line 3:
	''Back to [[e-Notebook]]''		''Back to [[e-Notebook]]''
	== Experiments ==		== Experiments ==
		+
		+	* Dilutions of pT luxI.cfp made for equivalence.
		+
		+	* pT.luxI.cfp inoculated into LB for Open Loop[A] (morning)
		+
		+	* pT.luxI.cfp and ''K12Z1'' inoculated in LB for Open Loop[B] (evening)
		+
		+	* pLac.cfp Inoculated for equivalence.
		+
		+	* Inoculated into 50 mL Glu M9 with 20 ng/mL aTc    (20*50=1 ug == 100 uL of 10 ug/mL)
		+	pT LuxI.C: 2 flasks - 1 ul/mL == 50 uL
		+	[A]                              - 0.1 ul/mL == 5 uL    (Krishna)
		+	K12z1:    1 flask  - 2 ul/mL == 100 uL
		+
		+	* Inoculated pL luxR yfp.pR cfp and ''K12Z1'' in LB for Open Loop[A]
		+
		+	* pLac.Cfp dilutions done (14+2+4=20 tubes)
		+
		+
		+
		+	'''Microscopy'''
		+
		+	* OD was measured and pT luxI.C imaging done for equivalence.
		+
		+	* pL.luxR.yfp imaging done for equivalence.

---

Revision as of 11:36, 29 June 2007

Back to Bangalore

Back to e-Notebook

Experiments

• Dilutions of pT luxI.cfp made for equivalence.

• pT.luxI.cfp inoculated into LB for Open Loop[A] (morning)

• pT.luxI.cfp and K12Z1 inoculated in LB for Open Loop[B] (evening)

• pLac.cfp Inoculated for equivalence.

• Inoculated into 50 mL Glu M9 with 20 ng/mL aTc (20*50=1 ug == 100 uL of 10 ug/mL)

            pT LuxI.C: 2 flasks - 1 ul/mL == 50 uL  

[A] - 0.1 ul/mL == 5 uL (Krishna)

            K12z1:     1 flask  - 2 ul/mL == 100 uL 

• Inoculated pL luxR yfp.pR cfp and K12Z1 in LB for Open Loop[A]

• pLac.Cfp dilutions done (14+2+4=20 tubes)

Microscopy

• OD was measured and pT luxI.C imaging done for equivalence.

• pL.luxR.yfp imaging done for equivalence.