June 27
From 2007.igem.org
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* Inoculated into 50 mL Glu M9 with 20 ng/mL aTc (20*50=1 ug == 100 uL of 10 ug/mL) | * Inoculated into 50 mL Glu M9 with 20 ng/mL aTc (20*50=1 ug == 100 uL of 10 ug/mL) | ||
| - | + | {| width="40%" | |
| - | + | |- | |
| - | + | | | |
| - | + | pT LuxI.C: 2 flasks - 1 ul/mL == 50 uL | |
| + | 0.1 ul/mL == 5 uL | ||
| + | |||
| + | K12z1: 1 flask - 2 ul/mL == 100 uL | ||
| + | |} | ||
| + | |||
* Inoculated pL luxR yfp.pR cfp and ''K12Z1'' in LB for Open Loop[A] | * Inoculated pL luxR yfp.pR cfp and ''K12Z1'' in LB for Open Loop[A] | ||
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* pL.luxR.yfp imaging done for equivalence. | * pL.luxR.yfp imaging done for equivalence. | ||
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| + | |||
| + | ==Analysis== | ||
Latest revision as of 12:10, 29 June 2007
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Experiments
- Dilutions of pT luxI.cfp made for equivalence.
- pT.luxI.cfp inoculated into LB for Open Loop[A] (morning)
- pT.luxI.cfp and K12Z1 inoculated in LB for Open Loop[B] (evening)
- pLac.cfp Inoculated for equivalence.
- Inoculated into 50 mL Glu M9 with 20 ng/mL aTc (20*50=1 ug == 100 uL of 10 ug/mL)
pT LuxI.C: 2 flasks - 1 ul/mL == 50 uL
0.1 ul/mL == 5 uL
K12z1: 1 flask - 2 ul/mL == 100 uL
|
- Inoculated pL luxR yfp.pR cfp and K12Z1 in LB for Open Loop[A]
- pLac.Cfp dilutions done (14+2+4=20 tubes)
Microscopy
- OD was measured and pT luxI.C imaging done for equivalence.
- pL.luxR.yfp imaging done for equivalence.
