BerkiGEM2007-AustinConstructionFile26
From 2007.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
Digest ADtemp3c (BsaI/BamHI)<br> | Digest ADtemp3c (BsaI/BamHI)<br> | ||
Digest ADtemp5c (BsaI/BglII)<br> | Digest ADtemp5c (BsaI/BglII)<br> | ||
| - | Ligate, product is: | + | Ligate, product is: I716076 (Second best all around) |
<br> | <br> | ||
Revision as of 22:53, 30 June 2007
Beta portion
pcr CA1154F/G00101 on 9145-I716059 (575 bp)
Product is ADtemp1c
Sub ADtemp1c into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2c
pcr CA1099F/D58 on ADtemp2c (3465 bp)
Product is ADtemp3c
_______________________________________________________
Alpha portion
Pcr CA1150F/CA1099R on I716062 (1727 bp)
Product is ADtemp5c
____________________________________________________________
Digest ADtemp3c (BsaI/BamHI)
Digest ADtemp5c (BsaI/BglII)
Ligate, product is: I716076 (Second best all around)
