Boston University/Project Progress
From 2007.igem.org
(Difference between revisions)
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[[Boston_University/Primer Design | Primer Design]] | [[Boston_University/Primer Design | Primer Design]] | ||
- | [[Boston_University/Shewy Competence and Transformation| Making Shewanella Competent and Transforming Plasmid]] | + | [[Boston_University/Shewy Competence and Transformation | Making Shewanella Competent and Transforming Plasmid]] |
== Materials We Need == | == Materials We Need == | ||
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EDIT THE WIIIIKIIII | EDIT THE WIIIIKIIII | ||
- | [[Boston_University | Back]] | + | == Protocols == |
+ | [[Boston_University/Heat Shock Protocol | Calcium Chloride/Heat Shock Plasmid Transformations Protocol]] | ||
+ | |||
+ | [[Boston_University/Filter Conjugation Protocol | Filter Cojugation Protocol]] | ||
+ | |||
+ | [[Boston_University/TOPO Protocol | pTrcHis TOPO TA Expression Kit Cloning Protocol]] | ||
+ | |||
+ | [[Boston_University/NEBCutter Protocl| Using NEBCutter for checking specific restriction enzymes against a sequence]] | ||
+ | |||
+ | [[Boston_University/Gel Protocol | Making an Electrophoresis Gel]] | ||
+ | |||
+ | [[Boston_University/Zymo Protocol | Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB | ||
+ | ]] | ||
+ | |||
+ | == Question and Answer == | ||
+ | |||
+ | [[Boston_University/Project Progress | Back]] |
Revision as of 17:08, 9 July 2007
Contents |
What We've Accomplished
Making Shewanella Competent and Transforming Plasmid
Materials We Need
Error-Prone PCR: From CAB(?)
Ligases: Need to Buy
Short-Term To-Do List
EDIT THE WIIIIKIIII
Protocols
Calcium Chloride/Heat Shock Plasmid Transformations Protocol
pTrcHis TOPO TA Expression Kit Cloning Protocol
Using NEBCutter for checking specific restriction enzymes against a sequence
Procedure for 50 mL E. Coli mixture in Zymo Broth or SOB
Question and Answer
Back