Melbourne/BBa B0014
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[[Melbourne/Parts used|<Return to Parts Used>]] [[Melbourne/Lab Notebook|<Return to Lab notebook>]] [[Melbourne|<Return to team homepage>]] | [[Melbourne/Parts used|<Return to Parts Used>]] [[Melbourne/Lab Notebook|<Return to Lab notebook>]] [[Melbourne|<Return to team homepage>]] | ||
=Name: Double Terminator= | =Name: Double Terminator= | ||
| + | Last Updated: 10:20am 09/07/07 | ||
==Physical DNA== | ==Physical DNA== | ||
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**Location: Cold Room | **Location: Cold Room | ||
**Miniprepped: 5/7 | **Miniprepped: 5/7 | ||
| - | ***Location: | + | ***Location: -20 freezer |
| - | **Confirmed: | + | ***Confirmed: Yes |
| - | **Glycerol Stock: | + | **Glycerol Stock: 5/7 |
| - | ***Location: | + | ***Location: -80 freezer |
==Expected Digestion Products== | ==Expected Digestion Products== | ||
| + | {| border="1" | ||
| + | |+ Expected Digestion Products | ||
| + | ! Digest !! Fragment 1 !! Fragment 2 | ||
| + | |- | ||
| + | ! EcoRI/PstI | ||
| + | | 131 || 3153 | ||
| + | |- | ||
| + | ! EcoRI/HaeII | ||
| + | | 579 || 2705 | ||
| + | |} | ||
| + | |||
__NOTOC__ | __NOTOC__ | ||
Latest revision as of 05:07, 10 July 2007
<Return to Parts Used> <Return to Lab notebook> <Return to team homepage>
Name: Double Terminator
Last Updated: 10:20am 09/07/07
Physical DNA
- Registry: BBa_0014
- Kit location: P1 1G
- Resistance: Amp, Kan
- Part Size: 95
- Plasmid Vector: pSB1AK3
- Vector Size: 3189
- Total Size: 3284
Status
- Labelling: P1 1G
- Resuspended: Yes
- Location: -20 freezer
- Transformed: Yes
- Location: Cold Room
- Miniprepped: 5/7
- Location: -20 freezer
- Confirmed: Yes
- Glycerol Stock: 5/7
- Location: -80 freezer
Expected Digestion Products
| Digest | Fragment 1 | Fragment 2 |
|---|---|---|
| EcoRI/PstI | 131 | 3153 |
| EcoRI/HaeII | 579 | 2705 |
