Paris/July 12
From 2007.igem.org
(Difference between revisions)
David.bikard (Talk | contribs) |
David.bikard (Talk | contribs) |
||
| Line 1: | Line 1: | ||
David B: | David B: | ||
| - | Preparation of a stock of phage on w121: | + | '''Preparation of a stock of phage on w121:''' |
* Filtration of the Cacl2 and MgSO4 stocks | * Filtration of the Cacl2 and MgSO4 stocks | ||
* Launch a culture: | * Launch a culture: | ||
Revision as of 09:07, 12 July 2007
David B:
Preparation of a stock of phage on w121:
- Filtration of the Cacl2 and MgSO4 stocks
- Launch a culture:
- 15ml LB
- 225µl Cacl2
- 450µl MgSO4
- 90µl DAP
Let grow until OD=0.2
