Melbourne/cannon plasmid pNL26 res map
From 2007.igem.org
(Difference between revisions)
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Restriction map of core enzymes from: http://arbl.cvmbs.colostate.edu/molkit/mapper/index.html | Restriction map of core enzymes from: http://arbl.cvmbs.colostate.edu/molkit/mapper/index.html | ||
+ | |||
{| border="1" | {| border="1" | ||
|- | |- | ||
!Enzyme !!Cuts !! --------- Base 5' to Cleave Sites ---------- | !Enzyme !!Cuts !! --------- Base 5' to Cleave Sites ---------- | ||
|- | |- | ||
- | |BamHI ||2 || | + | |BamHI ||2 ||2931 5043 |
|- | |- | ||
- | |BglII ||1 || | + | |BglII ||1 ||8448 |
|- | |- | ||
- | |ClaI || | + | |ClaI ||6 ||7 7314 7350 7708 9206 10014 |
|- | |- | ||
- | |EcoRI ||2 || | + | |EcoRI ||2 ||5799 10036 |
|- | |- | ||
- | |EcoRV ||2 || | + | |EcoRV ||2 ||9283 9501 |
|- | |- | ||
- | |HincII || | + | |HincII ||4 ||17 3662 4937 9767 |
|- | |- | ||
- | |HindIII ||3 || | + | |HindIII ||3 ||3479 4131 8983 |
|- | |- | ||
- | |KpnI ||2 || | + | |KpnI ||2 || 40 3418 |
|- | |- | ||
- | |NcoI ||2 || | + | |NcoI ||2 ||5306 5375 |
|- | |- | ||
- | |NotI ||1 || | + | |NotI ||1 ||2912 |
|- | |- | ||
- | |PstI ||7 || | + | |PstI ||7 ||2947 5463 5841 5946 9083 9116 9336 |
|- | |- | ||
- | |SacI ||2 || | + | |SacI ||2 ||2899 7920 |
|- | |- | ||
- | |SalI || | + | |SalI ||1 ||15 |
|- | |- | ||
- | |SmaI ||1 || | + | |SmaI ||1 ||2939 |
|- | |- | ||
- | |XbaI ||1 || | + | |XbaI ||1 || 2919 |
|- | |- | ||
- | |XhoI ||2 || | + | |XhoI ||2 ||21 5355 |
|} | |} |
Revision as of 05:49, 13 July 2007
Restriction map of core enzymes from: http://arbl.cvmbs.colostate.edu/molkit/mapper/index.html
Enzyme | Cuts | --------- Base 5' to Cleave Sites ---------- |
---|---|---|
BamHI | 2 | 2931 5043 |
BglII | 1 | 8448 |
ClaI | 6 | 7 7314 7350 7708 9206 10014 |
EcoRI | 2 | 5799 10036 |
EcoRV | 2 | 9283 9501 |
HincII | 4 | 17 3662 4937 9767 |
HindIII | 3 | 3479 4131 8983 |
KpnI | 2 | 40 3418 |
NcoI | 2 | 5306 5375 |
NotI | 1 | 2912 |
PstI | 7 | 2947 5463 5841 5946 9083 9116 9336 |
SacI | 2 | 2899 7920 |
SalI | 1 | 15 |
SmaI | 1 | 2939 |
XbaI | 1 | 2919 |
XhoI | 2 | 21 5355 |