Edinburgh/DivisionPopper
From 2007.igem.org
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=== Bacterial counter === | === Bacterial counter === | ||
| - | The idea is to use | + | The idea is to use a varity of recombinases to delete or invert sections of DNA and use this to count or trigger the expression of genes. |
'''Project Goal''' | '''Project Goal''' | ||
| - | The aim is to produce output as a function of cell division. This can have the added potential of performing programmed cell death after a determined number of divisions. We also hope to further analyse cell division and recombinase mechanisms. | + | The aim is to produce output as a function of cell division. This can have the added potential of performing programmed cell death after a determined number of cell divisions. We also hope to further analyse cell division and recombinase mechanisms. |
'''Current Device Idea''' | '''Current Device Idea''' | ||
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This can then be hooked up to another device such as a counter. | This can then be hooked up to another device such as a counter. | ||
| - | This device realise on | + | This device realise on dif sites flipping only once per division. To test whether this actually happens or not we will build this smaller, simpler device to prove flipping occurs upon cell division and a second test to show whether it flips once or many times per division |
Revision as of 15:45, 13 July 2007
https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG
Bacterial counter
The idea is to use a varity of recombinases to delete or invert sections of DNA and use this to count or trigger the expression of genes.
Project Goal
The aim is to produce output as a function of cell division. This can have the added potential of performing programmed cell death after a determined number of cell divisions. We also hope to further analyse cell division and recombinase mechanisms.
Current Device Idea
This device should output a PoPS signal every time a cell division occurs This can then be hooked up to another device such as a counter.
This device realise on dif sites flipping only once per division. To test whether this actually happens or not we will build this smaller, simpler device to prove flipping occurs upon cell division and a second test to show whether it flips once or many times per division
