BerkiGEM2007-HannahConstructionFile1

From 2007.igem.org

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Grow BBAI716316 in Lefty strain. Grow BCA1092 in Righty Strain.
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PCR HNX-F and HNX-R on H-Nox  (567bp, BglII/XhoI)
 
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Sub into pBca1144              ( BglII/XhoI, 2054+910  L)
 
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Product is pBca1144-H-NOX
 
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HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG
 
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HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA
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Digest BBA_I716316 with EcoRI and BamHI. Digest BCA1092 with EcoRI BglII.
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Ligate together.

Revision as of 18:30, 13 July 2007

Grow BBAI716316 in Lefty strain. Grow BCA1092 in Righty Strain.


Digest BBA_I716316 with EcoRI and BamHI. Digest BCA1092 with EcoRI BglII.

Ligate together.