Experiments

The following is the record of all the experiments done by us, each followed by graphs obtained by analysis of the corresponding microscopy and flow cytometry data.

In flow cytometry a signal obtained from a filter does not exactly correspond to CFP or YFP amount inside a cell; when cells express both the proteins. This is because of spectral overlap of their excitation and emission spectra. We came up with a mathematical method to separate CFP and YFP from autofluorescence and noise. The details about this mathematical tool for correction can be found here.