Paris/July 16
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== Plasmid pKs::DGAT expression in E. Coli == | == Plasmid pKs::DGAT expression in E. Coli == | ||
| - | + | We tried to see TG with NR died on E. Coli cells transfected with pKs::DGAT, an IPTG-inducible promoter. | |
| - | We tried | + | We tried different growth media containing more or less oleate, that should in theory increase TG synthesis.<br> |
| + | [[Image:Paris_EColi_DGAT.jpg]]<br> | ||
| + | Results : we don't see the inductible effect of IPTG. We can think that : | ||
| + | * Either the fluorescence without IPTG is due to a leak of the promoter | ||
| + | * Either DGAT is not induce in presence of IPTG, and the fluorescence we see is only a background. | ||
Revision as of 14:33, 16 July 2007
Plasmid pKs::DGAT expression in E. Coli
We tried to see TG with NR died on E. Coli cells transfected with pKs::DGAT, an IPTG-inducible promoter.
We tried different growth media containing more or less oleate, that should in theory increase TG synthesis.
Results : we don't see the inductible effect of IPTG. We can think that :
- Either the fluorescence without IPTG is due to a leak of the promoter
- Either DGAT is not induce in presence of IPTG, and the fluorescence we see is only a background.
