Boston University/Zymo Transformation Results

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{| style="width:700pt;" border="1"
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::::'''E. Coli Transformants'''
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:The image immediately to the right of this text shows the results from our transformation of E.coli with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
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:The contents of the plates are as follows(column indicates transformation type):
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{| align="center" border="1"
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|'''Zymo(poured)'''||'''Zymo(streaked)'''||'''Electroporation'''
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|-
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|pBAD18s|||pBAD18s|||pBAD18s
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|-
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|pBAD30|||pBAD30|||pBAD30
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|-
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|pjQ200|||pjQ200|||pjQ200
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|}
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:The "poured" and "streaked" designations refer to how the transformed E. coli were put onto the plate. When transformed E. coli were streaked onto the plate, 5 microliters were used. When transformed E. coli were poured onto the plate, 100 microliters were used.
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[[Image:BU_ecoli_zymo_electro.jpg]]
[[Image:BU_ecoli_zymo_electro.jpg]]
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[[Image:BU_shewy_zymo_electro.jpg]]
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|-
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|
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::::'''Shewy Transformants'''
 +
:The image immediately to the right of this text shows the results from our transformation of S. oneidensis with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
 +
 
 +
:The contents of the plates are as follows(column indicates transformation type):
 +
 
 +
{| align="center" border="1"
 +
|'''Zymo(streaked)'''||'''Zymo(poured)'''||'''Electroporation'''
 +
|-
 +
|pBAD18s|||pBAD18s|||pBAD18s
 +
|-
 +
|pBAD30|||pBAD30|||pBAD30
 +
|-
 +
|pjQ200|||pjQ200|||pjQ200
 +
|}
 +
 
 +
:The "poured" and "streaked" designations refer to how the transformed Shewy were put onto the plate. When transformed Shewy were streaked onto the plate, 5 microliters were used. When transformed Shewy were poured onto the plate, 100 microliters were used.
 +
 
 +
|[[Image:BU_shewy_zymo_electro.jpg]]
 +
|}

Revision as of 16:46, 16 July 2007

E. Coli Transformants
The image immediately to the right of this text shows the results from our transformation of E.coli with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
The contents of the plates are as follows(column indicates transformation type):
Zymo(poured)Zymo(streaked)Electroporation
pBAD18spBAD18spBAD18s
pBAD30pBAD30pBAD30
pjQ200pjQ200pjQ200
The "poured" and "streaked" designations refer to how the transformed E. coli were put onto the plate. When transformed E. coli were streaked onto the plate, 5 microliters were used. When transformed E. coli were poured onto the plate, 100 microliters were used.

BU ecoli zymo electro.jpg

Shewy Transformants
The image immediately to the right of this text shows the results from our transformation of S. oneidensis with plasmids pBAD18s, pBAD30, and pjQ200 via Zymo transformation and electroporation.
The contents of the plates are as follows(column indicates transformation type):
Zymo(streaked)Zymo(poured)Electroporation
pBAD18spBAD18spBAD18s
pBAD30pBAD30pBAD30
pjQ200pjQ200pjQ200
The "poured" and "streaked" designations refer to how the transformed Shewy were put onto the plate. When transformed Shewy were streaked onto the plate, 5 microliters were used. When transformed Shewy were poured onto the plate, 100 microliters were used.
BU shewy zymo electro.jpg


BU zymo pour ecoli.jpg BU zymo pour ecoli pjq200.jpg BU zymo pour shewy.jpg BU zymo streak ecoli.jpg BU zymo streak shewy.jpg

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