BerkiGEM2007-KristinConstructionFile12
From 2007.igem.org
(Difference between revisions)
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Composite part of RBS (pBca1101-1106A) + hemA from CFT073 | Composite part of RBS (pBca1101-1106A) + hemA from CFT073 | ||
- | Digest pBca1101-1106A | + | Digest pBca1101-1106A (BamH1/XhoI, 2132+850, L) |
Sub into pBca9145-I716152 (Bg1II/XhoI, 2063+1272, S) | Sub into pBca9145-I716152 (Bg1II/XhoI, 2063+1272, S) | ||
Product is pBca9145-I716162 | Product is pBca9145-I716162 | ||
</pre> | </pre> |
Latest revision as of 17:09, 17 July 2007
Composite part of RBS (pBca1101-1106A) + hemA from CFT073 Digest pBca1101-1106A (BamH1/XhoI, 2132+850, L) Sub into pBca9145-I716152 (Bg1II/XhoI, 2063+1272, S) Product is pBca9145-I716162