Template:Paris PCR 0
From 2007.igem.org
(Difference between revisions)
Nicolas C. (Talk | contribs) |
Nicolas C. (Talk | contribs) |
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|style="background: #ccccff;"| Name | |style="background: #ccccff;"| Name | ||
- | |style="background: #ccffcc;" colspan = "2" |{{{ | + | |style="background: #ccffcc;" colspan = "2" |{{{Name}}} |
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|style="background: #ccccff;"| Expected size | |style="background: #ccccff;"| Expected size | ||
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|style="background: #ccccff;"| Buffer (5x) | |style="background: #ccccff;"| Buffer (5x) | ||
- | | style="background: #ccffcc;" colspan = "2" |{{{ | + | | style="background: #ccffcc;" colspan = "2" |{{{Buffer}}} |
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- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{Size}}} |
|- | |- | ||
- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{Annealing}}} |
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|style="background: #ccccff;"| MgCl<sub>2</sub> 10µM | |style="background: #ccccff;"| MgCl<sub>2</sub> 10µM | ||
- | | style="background: #ccffcc;" colspan = "2" |{{{ | + | | style="background: #ccffcc;" colspan = "2" |{{{MgCl2}}} |
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|style="background: #ccccff;"| Success | |style="background: #ccccff;"| Success | ||
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|style="background: #ccccff;"| dNTP 10µM | |style="background: #ccccff;"| dNTP 10µM | ||
- | | style="background: #ccffcc;" colspan = "2" |{{{ | + | | style="background: #ccffcc;" colspan = "2" |{{{dNTP}}} |
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- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{Success}}} |
|- | |- | ||
- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{Elongation}}} |
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|style="background: #ccccff;"| Oligo F 10µM | |style="background: #ccccff;"| Oligo F 10µM | ||
- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{n_oligoF}}} |
- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{v_oligoF}}} |
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|style="background: #ccccff;"| Image (click to enlarge) | |style="background: #ccccff;"| Image (click to enlarge) | ||
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|style="background: #ccccff;"| Oligo R 10µM | |style="background: #ccccff;"| Oligo R 10µM | ||
- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{n_oligoR}}} |
- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{v_oligoR}}} |
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|style="background: #ccffcc;" rowspan| [[Image:{{{image}}}|30px]] | |style="background: #ccffcc;" rowspan| [[Image:{{{image}}}|30px]] | ||
|- | |- | ||
- | |style="background: #ccffcc;"| {{{ | + | |style="background: #ccffcc;"| {{{Cycles}}} |
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|style="background: #ccccff;"| Water | |style="background: #ccccff;"| Water | ||
- | | style="background: #ccffcc;" colspan = "2" |{{{ | + | | style="background: #ccffcc;" colspan = "2" |{{{Water}}} |
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|style="background: #ccccff;"| Band (0=ladder) | |style="background: #ccccff;"| Band (0=ladder) | ||
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|style="background: #ccccff;"| DNA | |style="background: #ccccff;"| DNA | ||
- | | style="background: #ccffcc;" colspan = "2" |{{{ | + | | style="background: #ccffcc;" colspan = "2" |{{{DNA}}} |
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|} | |} |
Revision as of 16:36, 18 July 2007
PCR : {{{Title}}} | ||||||
---|---|---|---|---|---|---|
PCR Settings | Name | {{{Name}}} | Expected size | |||
Annealing (°C) | Buffer (5x) | {{{Buffer}}} | {{{Size}}} | |||
{{{Annealing}}} | MgCl2 10µM | {{{MgCl2}}} | Success | |||
Time Elongation | dNTP 10µM | {{{dNTP}}} | {{{Success}}} | |||
{{{Elongation}}} | Oligo F 10µM | {{{n_oligoF}}} | {{{v_oligoF}}} | Image (click to enlarge) | ||
Number cycles | Oligo R 10µM | {{{n_oligoR}}} | {{{v_oligoR}}} | [[Image:{{{image}}}|30px]] | ||
{{{Cycles}}} | Water | {{{Water}}} | Band (0=ladder) | |||
Comments | Polymerase | {{{pol}}} | {{{band}}} | |||
{{{comment}}} | DNA | {{{DNA}}} |