Template:Paris PCR 0

From 2007.igem.org

(Difference between revisions)
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|style="background: #ccccff;"| Name
|style="background: #ccccff;"| Name
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|style="background: #ccffcc;" colspan = "2" |{{{name}}}
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|style="background: #ccffcc;" colspan = "2" |{{{Name}}}
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|style="background: #ccccff;"| Expected size
|style="background: #ccccff;"| Expected size
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|style="background: #ccccff;"| Buffer (5x)
|style="background: #ccccff;"| Buffer (5x)
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| style="background: #ccffcc;" colspan = "2" |{{{buffer}}}
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| style="background: #ccffcc;" colspan = "2" |{{{Buffer}}}
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|style="background: #ccffcc;"| {{{size}}}
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|style="background: #ccffcc;"| {{{Size}}}
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|style="background: #ccffcc;"| {{{annealing}}}
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|style="background: #ccffcc;"| {{{Annealing}}}
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|style="background: #ccccff;"| MgCl<sub>2</sub> 10µM
|style="background: #ccccff;"| MgCl<sub>2</sub> 10µM
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| style="background: #ccffcc;" colspan = "2" |{{{mgcl2}}}
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| style="background: #ccffcc;" colspan = "2" |{{{MgCl2}}}
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|style="background: #ccccff;"| Success
|style="background: #ccccff;"| Success
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|style="background: #ccccff;"| dNTP 10µM
|style="background: #ccccff;"| dNTP 10µM
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| style="background: #ccffcc;" colspan = "2" |{{{dntp}}}
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| style="background: #ccffcc;" colspan = "2" |{{{dNTP}}}
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|style="background: #ccffcc;"| {{{success}}}
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|style="background: #ccffcc;"| {{{Success}}}
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|style="background: #ccffcc;"| {{{elongation}}}
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|style="background: #ccffcc;"| {{{Elongation}}}
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|style="background: #ccccff;"| Oligo F 10µM
|style="background: #ccccff;"| Oligo F 10µM
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|style="background: #ccffcc;"| {{{n_oligo_f}}}
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|style="background: #ccffcc;"| {{{n_oligoF}}}
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|style="background: #ccffcc;"| {{{v_oligo_f}}}
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|style="background: #ccffcc;"| {{{v_oligoF}}}
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|style="background: #ccccff;"| Image (click to enlarge)
|style="background: #ccccff;"| Image (click to enlarge)
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|style="background: #ccccff;"| Oligo R 10µM
|style="background: #ccccff;"| Oligo R 10µM
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|style="background: #ccffcc;"| {{{n_oligo_r}}}
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|style="background: #ccffcc;"| {{{n_oligoR}}}
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|style="background: #ccffcc;"| {{{v_oligo_r}}}
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|style="background: #ccffcc;"| {{{v_oligoR}}}
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|style="background: #ccffcc;" rowspan| [[Image:{{{image}}}|30px]]
|style="background: #ccffcc;" rowspan| [[Image:{{{image}}}|30px]]
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|style="background: #ccffcc;"| {{{cycles}}}
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|style="background: #ccffcc;"| {{{Cycles}}}
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|style="background: #ccccff;"| Water
|style="background: #ccccff;"| Water
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| style="background: #ccffcc;" colspan = "2" |{{{water}}}
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| style="background: #ccffcc;" colspan = "2" |{{{Water}}}
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|style="background: #ccccff;"| Band (0=ladder)
|style="background: #ccccff;"| Band (0=ladder)
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|style="background: #ccccff;"| DNA
|style="background: #ccccff;"| DNA
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| style="background: #ccffcc;" colspan = "2" |{{{dna}}}
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| style="background: #ccffcc;" colspan = "2" |{{{DNA}}}
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Revision as of 16:36, 18 July 2007

PCR : {{{Title}}}
PCR Settings Name {{{Name}}} Expected size
Annealing (°C) Buffer (5x) {{{Buffer}}} {{{Size}}}
{{{Annealing}}} MgCl2 10µM {{{MgCl2}}} Success
Time Elongation dNTP 10µM {{{dNTP}}} {{{Success}}}
{{{Elongation}}} Oligo F 10µM {{{n_oligoF}}} {{{v_oligoF}}} Image (click to enlarge)
Number cycles Oligo R 10µM {{{n_oligoR}}} {{{v_oligoR}}} [[Image:{{{image}}}|30px]]
{{{Cycles}}} Water {{{Water}}} Band (0=ladder)
Comments Polymerase {{{pol}}} {{{band}}}
{{{comment}}} DNA {{{DNA}}}