Template:Paris PCR 0

From 2007.igem.org

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{|
{|
|- style="background: #ccccff;"
|- style="background: #ccccff;"
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! colspan="3" style="background: #ccccff;" | PCR : {{{Title}}}
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! colspan="7" style="background: #ccccff;" | PCR : {{{Title}}}
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
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|width= 33%|
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|width= 20%|
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|width= 33%|
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|width= 1%|
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|width= 33%|  
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|width= 5%|
-
|- style="background: #cccccc;"
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|width= 5%|
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| style="background: #ccffcc;" | Buffer (5x)
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|width= 5%|
-
|colspan="2" | {{{Buffer}}}
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|width= 1%|
-
|- style="background: #cccccc;"
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|width= 40%|
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| style="background: #ccffcc;" | MgCl<sub>2</sub> 10µM
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|-
-
|colspan="2" | {{{MgCl2}}}
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|style="background: #ffcccc;"| PCR Settings
-
|- style="background: #cccccc;"
+
|
-
| style="background: #ccffcc;" | dNTP 10µM
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|style="background: #ccccff;"| Buffer (5x)
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|colspan="2" | {{{dNTP}}}
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|style="background: #ccffcc;" colspan = "2" |{{{Buffer}}}
-
|- style="background: #cccccc;"
+
|
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| style="background: #ccffcc;" | oligoF 10µM
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|style="background: #ccccff;"| Expected size
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| {{{n_oligoF}}}
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|-
-
| {{{v_oligoF}}}
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|style="background: #ccccff;"| Annealing (°C)
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|- style="background: #cccccc;"
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|
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| style="background: #ccffcc;" | oligoR 10µM
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|style="background: #ccccff;"| MgCl<sub>2</sub> 10µM
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| {{{n_oligoR}}}
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| style="background: #ccffcc;" colspan = "2" |{{{MgCl2}}}
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| {{{v_oligoR}}}
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|
-
|- style="background: #cccccc;"
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|style="background: #ccffcc;"| {{{Size}}}
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| style="background: #ccffcc;" | water
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|-
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|colspan="2" | {{{water}}}
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|style="background: #ccffcc;"| {{{Annealing}}}
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|- style="background: #cccccc;"
+
|
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| style="background: #ccffcc;" | polymerase
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|style="background: #ccccff;"| dNTP 10µM
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|colspan="2" | {{{pol}}}
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| style="background: #ccffcc;" colspan = "2" |{{{dNTP}}}
-
|- style="background: #cccccc;"
+
|
-
| style="background: #ccffcc;" | DNA
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|style="background: #ccccff;"| Success
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|colspan="2" | {{{DNA}}}
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|-  
-
|- style="background: #cccccc;"
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|style="background: #ccccff;"| Time Elongation
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| style="background: #ccffcc;" | Expected Size kpb
+
|
-
|colspan="2" | {{{Size}}}
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|style="background: #ccccff;"| Oligo F 10µM
-
|- style="background: #cccccc;"
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|style="background: #ccffcc;"| {{{n_oligoF}}}
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| style="background: #ccffcc;" | Success
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|style="background: #ccffcc;"| {{{v_oligoF}}}
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|colspan="2" | {{{Success}}}
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|
 +
|style="background: #ccffcc;"| {{{Success}}}
 +
|-
 +
|style="background: #ccffcc;"| {{{Elongation}}}
 +
|
 +
|style="background: #ccccff;"| Oligo R 10µM
 +
|style="background: #ccffcc;"| {{{n_oligoR}}}
 +
|style="background: #ccffcc;"| {{{v_oligoR}}}
 +
|
 +
|style="background: #ccccff;"| Image (click to enlarge)
 +
|-  
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|style="background: #ccccff;"| Number cycles
 +
|
 +
|style="background: #ccccff;"| Water
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| style="background: #ccffcc;" colspan = "2" |{{{water}}}
 +
|
 +
|style="background: #ccffcc;" rowspan| [[Image:{{{Image}}}|30px]]
 +
|-
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|style="background: #ccffcc;"| {{{Cycles}}}
 +
|
 +
|style="background: #ccccff;"| Polymerase
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| style="background: #ccffcc;" colspan = "2" |{{{pol}}}
 +
|
 +
|style="background: #ccccff;"| Band (0=ladder)
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|-
 +
|
 +
|
 +
|style="background: #ccccff;"| DNA
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| style="background: #ccffcc;" colspan = "2" |{{{DNA}}}
 +
|
 +
|style="background: #ccffcc;"| {{{Band}}}
|}
|}

Latest revision as of 16:45, 18 July 2007

PCR : {{{Title}}}
PCR Settings Buffer (5x) {{{Buffer}}} Expected size
Annealing (°C) MgCl2 10µM {{{MgCl2}}} {{{Size}}}
{{{Annealing}}} dNTP 10µM {{{dNTP}}} Success
Time Elongation Oligo F 10µM {{{n_oligoF}}} {{{v_oligoF}}} {{{Success}}}
{{{Elongation}}} Oligo R 10µM {{{n_oligoR}}} {{{v_oligoR}}} Image (click to enlarge)
Number cycles Water {{{water}}} [[Image:{{{Image}}}|30px]]
{{{Cycles}}} Polymerase {{{pol}}} Band (0=ladder)
DNA {{{DNA}}} {{{Band}}}
Retrieved from "http://2007.igem.org/wiki/index.php/Template:Paris_PCR_0"