Template:Paris PCR 0

From 2007.igem.org

(Difference between revisions)
 
(6 intermediate revisions not shown)
Line 13: Line 13:
|style="background: #ffcccc;"| PCR Settings
|style="background: #ffcccc;"| PCR Settings
|
|
-
|style="background: #ccccff;"| Name
+
|style="background: #ccccff;"| Buffer (5x)
-
|style="background: #ccffcc;" colspan = "2" |{{{name}}}
+
|style="background: #ccffcc;" colspan = "2" |{{{Buffer}}}
|
|
|style="background: #ccccff;"| Expected size
|style="background: #ccccff;"| Expected size
Line 20: Line 20:
|style="background: #ccccff;"| Annealing (°C)
|style="background: #ccccff;"| Annealing (°C)
|
|
-
|style="background: #ccccff;"| Buffer (5x)
+
|style="background: #ccccff;"| MgCl<sub>2</sub> 10µM
-
| style="background: #ccffcc;" colspan = "2" |{{{buffer}}}
+
| style="background: #ccffcc;" colspan = "2" |{{{MgCl2}}}
|
|
-
|style="background: #ccffcc;"| {{{size}}}
+
|style="background: #ccffcc;"| {{{Size}}}
|-  
|-  
-
|style="background: #ccffcc;"| {{{annealing}}}
+
|style="background: #ccffcc;"| {{{Annealing}}}
|
|
-
|style="background: #ccccff;"| MgCl<sub>2</sub> 10µM
+
|style="background: #ccccff;"| dNTP 10µM
-
| style="background: #ccffcc;" colspan = "2" |{{{mgcl2}}}
+
| style="background: #ccffcc;" colspan = "2" |{{{dNTP}}}
|
|
|style="background: #ccccff;"| Success
|style="background: #ccccff;"| Success
|-  
|-  
|style="background: #ccccff;"| Time Elongation
|style="background: #ccccff;"| Time Elongation
-
|
 
-
|style="background: #ccccff;"| dNTP 10µM
 
-
| style="background: #ccffcc;" colspan = "2" |{{{dntp}}}
 
-
|
 
-
|style="background: #ccffcc;"| {{{success}}}
 
-
|-
 
-
|style="background: #ccffcc;"| {{{elongation}}}
 
|
|
|style="background: #ccccff;"| Oligo F 10µM
|style="background: #ccccff;"| Oligo F 10µM
-
|style="background: #ccffcc;"| {{{n_oligo_f}}}
+
|style="background: #ccffcc;"| {{{n_oligoF}}}
-
|style="background: #ccffcc;"| {{{v_oligo_f}}}
+
|style="background: #ccffcc;"| {{{v_oligoF}}}
|
|
-
|style="background: #ccccff;"| Image
+
|style="background: #ccffcc;"| {{{Success}}}
|-  
|-  
-
|style="background: #ccccff;"| Number cycles
+
|style="background: #ccffcc;"| {{{Elongation}}}
|
|
|style="background: #ccccff;"| Oligo R 10µM
|style="background: #ccccff;"| Oligo R 10µM
-
|style="background: #ccffcc;"| {{{n_oligo_r}}}
+
|style="background: #ccffcc;"| {{{n_oligoR}}}
-
|style="background: #ccffcc;"| {{{v_oligo_r}}}
+
|style="background: #ccffcc;"| {{{v_oligoR}}}
|
|
-
|style="background: #ccffcc;" rowspan| [[Image:{{{image}}}|30px]]
+
|style="background: #ccccff;"| Image (click to enlarge)
|-  
|-  
-
|style="background: #ccffcc;"| {{{cycles}}}
+
|style="background: #ccccff;"| Number cycles
|
|
|style="background: #ccccff;"| Water
|style="background: #ccccff;"| Water
| style="background: #ccffcc;" colspan = "2" |{{{water}}}
| style="background: #ccffcc;" colspan = "2" |{{{water}}}
|
|
-
|style="background: #ccccff;"| Band (0=ladder)
+
|style="background: #ccffcc;" rowspan| [[Image:{{{Image}}}|30px]]
|-  
|-  
-
|style="background: #ccccff;"| Comments
+
|style="background: #ccffcc;"| {{{Cycles}}}
-
|  
+
|
|style="background: #ccccff;"| Polymerase
|style="background: #ccccff;"| Polymerase
| style="background: #ccffcc;" colspan = "2" |{{{pol}}}
| style="background: #ccffcc;" colspan = "2" |{{{pol}}}
|
|
-
|style="background: #ccffcc;"| {{{band}}}
+
|style="background: #ccccff;"| Band (0=ladder)
|-  
|-  
-
|style="background: #ccffcc;"| {{{comment}}}
 
|
|
 +
|
|style="background: #ccccff;"| DNA
|style="background: #ccccff;"| DNA
-
| style="background: #ccffcc;" colspan = "2" |{{{dna}}}
+
| style="background: #ccffcc;" colspan = "2" |{{{DNA}}}
|
|
 +
|style="background: #ccffcc;"| {{{Band}}}
|}
|}

Latest revision as of 16:45, 18 July 2007

PCR : {{{Title}}}
PCR Settings Buffer (5x) {{{Buffer}}} Expected size
Annealing (°C) MgCl2 10µM {{{MgCl2}}} {{{Size}}}
{{{Annealing}}} dNTP 10µM {{{dNTP}}} Success
Time Elongation Oligo F 10µM {{{n_oligoF}}} {{{v_oligoF}}} {{{Success}}}
{{{Elongation}}} Oligo R 10µM {{{n_oligoR}}} {{{v_oligoR}}} Image (click to enlarge)
Number cycles Water {{{water}}} [[Image:{{{Image}}}|30px]]
{{{Cycles}}} Polymerase {{{pol}}} Band (0=ladder)
DNA {{{DNA}}} {{{Band}}}
Retrieved from "http://2007.igem.org/wiki/index.php/Template:Paris_PCR_0"