Paris/July 18
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| + | == Transduction of w121 strain == | ||
| + | Titration of the stock of phage from 12.7.7. : ~10<sup>9</sup> phages/ml : OK | ||
| + | <br> | ||
== FtsZ<sup>TS</sup> strain screening == | == FtsZ<sup>TS</sup> strain screening == | ||
We want to test these strains : | We want to test these strains : | ||
Revision as of 17:40, 18 July 2007
Contents |
Transduction of w121 strain
Titration of the stock of phage from 12.7.7. : ~109 phages/ml : OK
FtsZTS strain screening
We want to test these strains :
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3
I made glycerol stock of these strains, stored in the freezer. Test :
- Spread these strains on two plates (preheated before spreading) :
- One at 30°C
- One at 42°C
The results tomorrow.
Minipreps
We have 2 clones for each plasmid :
- E0422
- E0241
- E0840
- B0030
- J61047
Elution within 50µL H2O
DNA is within the Miniprep box in the -20°C fridge.
Digestion products
Photos !!!
DAP solution contamination test
Results :
- 50µL of H20 (control) OK
- 50µL of aliquot DAP 50mM (in use) OK
- 50µL of DAP 1mM CONTAMINATED
- 50µL of DAP 0.2 mM OK
- 50µL of DAP 50mM OK
