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	====Wed 18th July====		====Wed 18th July====
	* Digest GFP and mint mini preps and ran on gel		* Digest GFP and mint mini preps and ran on gel
		+
		+	====Thu 19th July====
		+	* Repeat digest of mini preps with more DNA

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Revision as of 10:13, 19 July 2007

https://static.igem.org/mediawiki/2007/f/f5/800px-Edinburgh_City_15_mod.JPG

Contents 1 Biobricks Revived 2 Lab Diary 2.1 Thu 12th July 2.2 Fri 13th July 2.3 Mon 16th July 2.4 Tue 17th July 2.5 Wed 18th July 2.6 Thu 19th July

Biobricks Revived

This is a list of the biobricks we have revived from the registry

Brick	Description	Well	Used for
[http://partsregistry.org/Part:BBa_J04430 BBa_J04430]	IPTG induced GFP	Plate 2, 1L	Test biobrick revival process
[http://partsregistry.org/Part:BBa_E0241 BBa_E0241]	PoPS --> GFP	Plate 2, 15L	For use in dif experement 1
[http://partsregistry.org/Part:BBa_J45700 BBa_J45700]	Wintergreen Scent	Plate 3, 7B	Place into lacto bacillus

Lab Diary

Thu 12th July

• PTG induced GFP revived ([http://partsregistry.org/Part:BBa_J04430 BBa_J04430])

Test biobrick revival process

Fri 13th July

• Alfalfa Seeds Planted (in Agar)

Growing alfalfa plants, to get leaves from which to extract DNA for the caffeic acid-3-o-methyltransferase gene (part of the vanillin synthesis pathway)

• Biobricks [http://partsregistry.org/Part:BBa_E0241 BBa_E0241] and [http://partsregistry.org/Part:BBa_J45700 BBa_J45700] rivived

Mon 16th July

• Begin DNA extraction from Bamboo

Test DNA extraction process from plants

• Prepared mini preps for GFP and mint genes

Tue 17th July

• Alfalfa Transferred to compost

• DNA containing GFP and mint genes extracted from E.Coli

Wed 18th July

• Digest GFP and mint mini preps and ran on gel

Thu 19th July

• Repeat digest of mini preps with more DNA