Bologna University/LB medium

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::1. To prepare 1 l of LB medium dissolve in ultrapure water:
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::1. To prepare 1 L of LB medium dissolve in ultrapure water:
:::::: -Tryptone 10 g
:::::: -Tryptone 10 g
:::::: -Yeast extract 5 g
:::::: -Yeast extract 5 g
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::2. Autoclave and store at room temperature.
::2. Autoclave and store at room temperature.
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[[Bologna University | Mediums and buffers]]
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[[Bologna University/Mediums and buffers | Back]]

Latest revision as of 09:23, 25 October 2007

1. To prepare 1 L of LB medium dissolve in ultrapure water:
-Tryptone 10 g
-Yeast extract 5 g
-NaCl 10 g
-5M NaOH 200 μl
2. Autoclave and store at room temperature.

Back