Melbourne/Plan/Red Photosensor
From 2007.igem.org
| (4 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
[[Melbourne/Plan|<return to top of plan>]] [[melbourne|<return to home page>]] [[Melb:Blue Photosensor|<next>]] | [[Melbourne/Plan|<return to top of plan>]] [[melbourne|<return to home page>]] [[Melb:Blue Photosensor|<next>]] | ||
| - | == | + | The Plan for the implementation of the blue light system into a functioning BioBrick |
| + | * D - Day | ||
| + | * N - Night | ||
| + | |||
| + | == Timeline == | ||
| + | Week 0 (week beginning 29/07/07) | ||
| + | * ligations ... | ||
| + | * craig starting to make test harness | ||
| + | |||
| + | |||
| + | Week 1 (week beginning 06/08/07) | ||
| + | |||
| + | * D1: Ligate Gen-RBS into LacZ | ||
| + | * D2: Transform onto +Gen,+Kan plates | ||
| + | * D3: Pick colonies and culture | ||
| + | * D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI | ||
| + | * N4: Ligate. We might be able to do a short ligation during the day. | ||
| + | * D5: Transform onto +Gen, +Kan plates | ||
| + | * D6: Pick colonies and culture | ||
| + | |||
| + | |||
| + | Week 2 (week beginning 06/08/07) | ||
| + | |||
| + | * D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP | ||
| + | * N8: Ligate | ||
| + | * D9: Transform onto +Amp plates | ||
| + | * D10: Pick colonies | ||
| + | * D11: Miniprep | ||
| + | * D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid | ||
| + | * D13: Transform into EnvZ- | ||
| + | * D14: Pick colonies | ||
| + | |||
| + | |||
| + | Week 3 (week beginning 13/08/07) | ||
| + | |||
| + | Transformation into EnvZ- | ||
| + | * D1: Make cells competent | ||
| + | * D2: Transform cells with construct. | ||
| + | |||
| + | Week 4 (week beginning 20/08/07) | ||
| + | |||
| + | |||
| + | Week 5 (week beginning 27/08/07) | ||
| + | * Rinse and repeat | ||
Latest revision as of 23:38, 6 August 2007
<return to top of plan> <return to home page> <next>
The Plan for the implementation of the blue light system into a functioning BioBrick
- D - Day
- N - Night
Timeline
Week 0 (week beginning 29/07/07)
- ligations ...
- craig starting to make test harness
Week 1 (week beginning 06/08/07)
- D1: Ligate Gen-RBS into LacZ
- D2: Transform onto +Gen,+Kan plates
- D3: Pick colonies and culture
- D4: Miniprep and digest with EcoRI/SpeI. Also digest term (can't remember part name) with EcoRI/XbaI
- N4: Ligate. We might be able to do a short ligation during the day.
- D5: Transform onto +Gen, +Kan plates
- D6: Pick colonies and culture
Week 2 (week beginning 06/08/07)
- D7: Miniprep and digest with XbaI/PstI. Gel purify. Also digest OmpR+ with SpeI/PstI + AP
- N8: Ligate
- D9: Transform onto +Amp plates
- D10: Pick colonies
- D11: Miniprep
- D12: Digest with XbaI/PstI and ligate behind Light receptor plasmid
- D13: Transform into EnvZ-
- D14: Pick colonies
Week 3 (week beginning 13/08/07)
Transformation into EnvZ-
- D1: Make cells competent
- D2: Transform cells with construct.
Week 4 (week beginning 20/08/07)
Week 5 (week beginning 27/08/07)
- Rinse and repeat
