McGill

From 2007.igem.org

(Difference between revisions)

Latest revision as of 03:57, 27 October 2007

The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada

Project Overview

Quorum-sensing coupled with the Repressilator

Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.

Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.

In the Lab

Lab Notebook
Lab Protocols
Background Papers

The Team

Team Members
Mailing List

Pictures

[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup] (External)
Rhode Island Meetup
McGill Research Conference

Please feel free to check out our work from last year at iGEM McGill 2006

@@ Line 1: / Line 1: @@
-== Message Board (temporary) ==
+[[Image:banner_1.gif]]
-I'll chop up Adam's PDF on my break today to put it into the Protocols section. '''Tim'''
+<center>The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada</center><br>
+{| cellspacing="2px" cellpadding="5" border="0" style="padding: 0; width: 935px; color: #000000; background-color: #ffffff;"
+| width=750px style="padding: 20px; background-color: #ffffff; border: 4px solid #FF0000;" |
-Hello Tim,
+==  ==
+<center>[[Image:McGillIgem2007team.jpg|center|400px]]
+<html>
+<style>
-we need a lab notebook page, protocols page and team members page, etc.
+ul#list-nav {
+list-style:none;
+margin:20px;
+padding:0;
-PS: Everyone, get a lab notebook! We're making our cells chemically competent tomorrow so if you want to see/help out then show up around 10:30. [[User:Horiavulpe|Horiavulpe]] 16:41, 14 May 2007 (EDT)
+}
+ul#list-nav li {
+display:inline
+}
-Note from iGEM: Please use the format "McGill/Page Name" for all the pages your team uses. I fixed
+ul#list-nav li a {
-teh Lab Protocols, the Lab Notebook, and the Team Roster links.  Thanks,  - Randy
+text-decoration:none;
+padding:10px 0;
+width:130px;
+background:#DB231E;
+color:#eee;
+float:left;
+text-align:center;
+border-left:1px solid #fff;
+}
+ul#list-nav li a:hover {
+background:#FFFFFF;
+color:#000
+}
+</style>
+<br>
+ <ul id="list-nav">
+<li><a href="https://2007.igem.org/McGill/Project_Description">Project</a></li>
+<li><a href="https://2007.igem.org/McGill/Modeling_and_Simulations">Simulations</a></li>
+<li><a href="https://2007.igem.org/McGill/Team_2_More_Information">Animations</a></li>
+<li><a href="https://2007.igem.org/McGill/Project_Overview">Future</a></li>
+<li><a href="https://2007.igem.org/McGill/Results">Results</a></li>
+<li><a href="https://2007.igem.org/McGill/Parts">Parts</a></li>
+</ul>
+</html>
+</center><br><br><br><br>
+== Project Overview ==
-== In the Lab ==
+<u>'''Quorum-sensing coupled with the Repressilator'''</u>
-[[McGill/Lab Protocols|Lab Protocols]]<br>
-[[McGill/Lab Notebook|Lab Notebook]]
+Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.
+Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.<br>
+<center>
+[[Image:2gene oscillator.jpg|320px]]<br>
+</center><br>
+<br>
+{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
+|-valign="top"
+|width=240px style="padding: 5px|
+==[[Image:bacteria1.jpg]] In the Lab==
+[[McGill/Lab Notebook|Lab Notebook]]<br>
+[[McGill/Lab Protocols|Lab Protocols]]<br>
+[[McGill/Team_2_Background_Papers|Background Papers]] <br>
-== The Team ==
-[[McGill/Mailing List|Mailing List]]<br>
-[[McGill/Team Roster| Team Roster]]
-== Project Overviews ==
-'''Team 1 - Repressilator'''
-Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pLac, hoping that this would assist in synchronizing the oscillations. We will also test the effect of extracellularly adding autoinducer on synchronization.<br>
-[[McGill/Background|Background]]
+|width=240px style="padding: 5px|
+==[[Image:greenbulb.jpg]] The Team ==
+[[McGill/Team Roster| Team Members]]<br>
+[[McGill/Mailing List|Mailing List]]
+|width=240px style="padding: 5px|
+==[[Image:sine.jpg]] Pictures ==
+[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup]   (External) <br>
+[[McGill/RI meetup|Rhode Island Meetup]]<br>
+[[McGill/urc|McGill Research Conference]]<br>
-'''Team 2 - Fluorescence Complimentation'''
+Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]
-This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence.  We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence.  These half proteins will then be attached to proteins that revesibly associate.<br>

Views

Personal tools