NYMU Taipei/Lab Notes/2007 8 9
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| - | * | + | * Transformation results |
| - | ** | + | **[http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : <font color="red">Only 1 colony</font> |
| + | **[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : <font color="red">8 colonies</font> | ||
| - | *Transformation to competent cell | + | *Pick single colony and incubate at LB plate |
| + | |||
| + | *Transformation to competent cell (Add <font color="red">2uL</font> vector to 50uL ECOS competent cells) | ||
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2 | **Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2 | ||
| - | **Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again) | + | **Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 <font color="red">(Transformation again)</font> |
Latest revision as of 13:13, 9 August 2007
- Transformation results
- [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR : Only 1 colony
- [http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription : 8 colonies
- Pick single colony and incubate at LB plate
- Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
- Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
- Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)
