McGill

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Latest revision as of 03:57, 27 October 2007

The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada

Project Overview

Quorum-sensing coupled with the Repressilator

Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.

Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.

In the Lab

Lab Notebook
Lab Protocols
Background Papers

The Team

Team Members
Mailing List

Pictures

[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup] (External)
Rhode Island Meetup
McGill Research Conference

Please feel free to check out our work from last year at iGEM McGill 2006

@@ Line 1: / Line 1: @@
-== Message Board (temporary) ==
+[[Image:banner_1.gif]]
-Guys, when you make the links for the wiki pages, please follow this example:
+<center>The official wiki of the McGill University iGEM 2007 team of Montreal, Quebec, Canada</center><br>
+{| cellspacing="2px" cellpadding="5" border="0" style="padding: 0; width: 935px; color: #000000; background-color: #ffffff;"
+| width=750px style="padding: 20px; background-color: #ffffff; border: 4px solid #FF0000;" |
-'''<nowiki>[[McGill/Link_to_page|Display Name]]</nowiki>'''
+==  ==
+<center>[[Image:McGillIgem2007team.jpg|center|400px]]
+<html>
+<style>
-An actual link would look like this in wiki code:
+ul#list-nav {
-'''<nowiki>[[McGill/Team_1:_Fluorescence_Complementation|Team 1: Fluorescence Complementation]]</nowiki>'''
+list-style:none;
+margin:20px;
+padding:0;
-Thanks! - '''Hanmo'''
+}
+ul#list-nav li {
+display:inline
+}
+ul#list-nav li a {
+text-decoration:none;
+padding:10px 0;
+width:130px;
+background:#DB231E;
+color:#eee;
+float:left;
+text-align:center;
+border-left:1px solid #fff;
+}
-I'll chop up Adam's PDF on my break today to put it into the Protocols section. '''Tim'''
+ul#list-nav li a:hover {
+background:#FFFFFF;
+color:#000
+}
+</style>
+<br>
+ <ul id="list-nav">
+<li><a href="https://2007.igem.org/McGill/Project_Description">Project</a></li>
+<li><a href="https://2007.igem.org/McGill/Modeling_and_Simulations">Simulations</a></li>
+<li><a href="https://2007.igem.org/McGill/Team_2_More_Information">Animations</a></li>
+<li><a href="https://2007.igem.org/McGill/Project_Overview">Future</a></li>
+<li><a href="https://2007.igem.org/McGill/Results">Results</a></li>
+<li><a href="https://2007.igem.org/McGill/Parts">Parts</a></li>
+</ul>
+</html>
+</center><br><br><br><br>
+== Project Overview ==
-Hello Tim,
+<u>'''Quorum-sensing coupled with the Repressilator'''</u>
-we need a lab notebook page, protocols page and team members page, etc.
+Our project is a continuation of one of the projects we presented last year: a two-gene oscillator with an 'On' and 'Off' switch using LacI and LuxI. This simple relaxation oscillator could be used as a modular component for the synchronization of other complex oscillating systems such as the repressilator.
-PS: Everyone, get a lab notebook! We're making our cells chemically competent tomorrow so if you want to see/help out then show up around 10:30. [[User:Horiavulpe|Horiavulpe]] 16:41, 14 May 2007 (EDT)
+Quorum-sensing coupling between cells is achieved with a diffusible autoinducer AI (AHL) made from LuxI which promotes the synthesis of LacI from pLux. As Lac accumulates, it represses pLac which controls the synthesis of LuxI.<br>
+<center>
+[[Image:2gene oscillator.jpg|320px]]<br>
+</center><br>
+<br>
+{| cellspacing="2px" cellpadding="20" border="0" style="padding: 0px; width: 750px
+|-valign="top"
+|width=240px style="padding: 5px|
+==[[Image:bacteria1.jpg]] In the Lab==
-Note from iGEM: Please use the format "McGill/Page Name" for all the pages your team uses. I fixed
+[[McGill/Lab Notebook|Lab Notebook]]<br>
-teh Lab Protocols, the Lab Notebook, and the Team Roster links.  Thanks,  - Randy
-== In the Lab ==
 [[McGill/Lab Protocols|Lab Protocols]]<br>
-[[McGill/Lab Notebook|Lab Notebook]]
+[[McGill/Team_2_Background_Papers|Background Papers]] <br>
-== The Team ==
-[[McGill/Mailing List|Mailing List]]<br>
-[[McGill/Team Roster| Team Roster]]
-== Project Overviews ==
-'''Team 1 - Repressilator'''
-Our project consists of a two-gene oscillator with coupling by an autoinducer (AI). This system was presented last year. We will test the effects of adding AI and Tetracycline to the medium. We will also investigate the nature of the oscillations under different cell densities.<br>
-[[McGill/Background|Background]]
+|width=240px style="padding: 5px|
+==[[Image:greenbulb.jpg]] The Team ==
+[[McGill/Team Roster| Team Members]]<br>
+[[McGill/Mailing List|Mailing List]]
+|width=240px style="padding: 5px|
+==[[Image:sine.jpg]] Pictures ==
+[http://www.flickr.com/photos/cumbers/archives/date-posted/2007/08/21/ Brown University Meetup]   (External) <br>
+[[McGill/RI meetup|Rhode Island Meetup]]<br>
+[[McGill/urc|McGill Research Conference]]<br>
-'''Team 2 - Fluorescence Complimentation'''
+Please feel free to check out our work from last year at [https://2006.igem.org/wiki/index.php/McGill_University iGEM McGill 2006]
-This project on fluorescence complementation involves confirming previous work using Jun and Fos heterodimerizing proteins, each fused to a half terminus of YFP. The two halves of the YFP protein bind, giving rise to fluorescence.  We will then carry out the experiment with GFP or YFP split at different points in order to find a cleavage point that produces reversible fluorescence.  These half proteins will then be attached to proteins that revesibly associate.<br>

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