NYMU Taipei/Part List

From 2007.igem.org

(Difference between revisions)
(→antibiotics keys)
(→Part List)
 
(5 intermediate revisions not shown)
Line 79: Line 79:
|2
|2
|OmpR binding sites
|OmpR binding sites
-
|
+
|65
|
|
|?
|?
Line 117: Line 117:
|This part accepts POPS to generate EYFP under the medium strength RBS B0032
|This part accepts POPS to generate EYFP under the medium strength RBS B0032
|742
|742
 +
|pSB1A2
 +
|OK
 +
|-
 +
|[http://partsregistry.org/Part:BBa_E0840 BBa_E0840]
 +
|B0030(RBS).E0040(GFP).B0015(D-term)
 +
|16E, plate 1
 +
|gfp tri-part; strong rbs
 +
|878
|pSB1A2
|pSB1A2
|OK
|OK
Line 158: Line 166:
* A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
* A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
-
** stock (100mg/mL = 100ug/uL) ampicilin
+
** stock (100 mg/mL = 100 ug/uL) ampicillin
-
** liquid culture (50ug/mL = 250ug/5mL)
+
** liquid culture (50 ug/mL = 250 ug/5mL)
-
** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicilin
+
** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicillin
* K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
* K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
* C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
* C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
-
** 20mg/mL in Ethanol
+
** 35 mg/mL
 +
** 20 mg/mL in Ethanol
** we prepared 100mg in 5 mL and stored in -20 C
** we prepared 100mg in 5 mL and stored in -20 C

Latest revision as of 09:08, 24 January 2008

Project Description / Lab Notes / Part List / Primer List

Part List

Series alias Location Description part length vector (antibotics) Check
[http://partsregistry.org/Part:BBa_R0082 BBa_R0082] pOmpC 1 Positively regulated, OmpR-controlled promoter 108 pSB1A2 ?
[http://partsregistry.org/Part:BBa_C0082 BBa_C0082] tar-envZ 1 Receptor, tar-envZ 1491 pSB2K3
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034] RBS 1,2,3 The Elowitz RBS (Ribosome binding site) is the definition of efficiency 1.0 12 pSB1A2 ?
AU43-C7 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=13528923 INS] human insulin 495 C
INS_A 1 alpha chain of human insulin 63
INS_B 1 beta chain of human insulin 90
[http://partsregistry.org/Part:BBa_B0014 BBa_B0014] D-term 1,2,3 Double transcription terminator 95 pSB1AK3 OK
[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] pLacIQ 2 Constitutive Promoter, High Transcription 37 pSB2K3
OmpRBS 2 OmpR binding sites 65 ?
AV88-C6 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27371195 IDE] 2 insulin degradation enzyme of mouse 3445 A
AU61-F6 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=33879043 IGFBP3] 2 Insulin-like growth factor binding protein 3 of human 2488 C
AU50-C7 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=38114635 IGFBP7] 2 Insulin-like growth factor binding protein 7 of human 1155 C
[http://partsregistry.org/Part:BBa_I15016 BBa_I15016] B0032.ECFP 16D, plate 1 This part accepts POPS to produce ECFP under the medium RBS B0032 742 pSB1A2 OK
[http://partsregistry.org/Part:BBa_I15017 BBa_I15017] B0032.EYFP 16F, plate 1 This part accepts POPS to generate EYFP under the medium strength RBS B0032 742 pSB1A2 OK
[http://partsregistry.org/Part:BBa_E0840 BBa_E0840] B0030(RBS).E0040(GFP).B0015(D-term) 16E, plate 1 gfp tri-part; strong rbs 878 pSB1A2 OK
[http://partsregistry.org/Part:BBa_R0077 BBa_R0077] pCinRHSL, RBS+ 17L, Plate 1 CinR-HSL complex binds to this promoter and activates transcription. This part contains RBS. 231 pSB1A2 OK
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078] pCinRHSL 15D, Plate 1 CinR-HSL complex binds to this promoter and activates transcription. This part DOES NOT contain RBS. 225 pSB1A2 ?
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077] CinR 7G, Plate 2 cinR activator 762 pSB2K3 OK
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076] HSL 7E, Plate 2 autoinducer 702 pSB2K3 OK

antibiotics keys

  • A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
    • refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
    • stock (100 mg/mL = 100 ug/uL) ampicillin
    • liquid culture (50 ug/mL = 250 ug/5mL)
    • 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicillin
  • K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
  • C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
    • 35 mg/mL
    • 20 mg/mL in Ethanol
    • we prepared 100mg in 5 mL and stored in -20 C
Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Part_List"