From 2007.igem.org

Revision as of 10:10, 1 September 2007 (view source) Lihsiangyen (Talk | contribs) ← Older edit		Latest revision as of 05:56, 16 September 2007 (view source) Blent (Talk | contribs)
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Line 10:		Line 10:
	**T4 ligase: 1ul		**T4 ligase: 1ul
	**Incubate at RT for 3h		**Incubate at RT for 3h
-	* Plate Culture (16h)	+	* Plate Culture and Transformation(16h)
	**DNA: 2ul		**DNA: 2ul
	**Competent cell: 50ul		**Competent cell: 50ul
Line 17:		Line 17:
	* Plasmid Extraction (1hr)		* Plasmid Extraction (1hr)
	**Qiagene Miniprep Kit		**Qiagene Miniprep Kit
-	*Enzyme digestion to ligation formula	+	* Enzyme digestion to ligation formula
-	**	+	** [[NYMU Taipei/phptest | phptest]]

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Latest revision as of 05:56, 16 September 2007

• Enzyme Digestion (2h)
  • Restriction enzymes: 1ul each
  • Incubate for 2h
  • Heat inactivation at 65°C, 20min
• Gel Separation (0.5h)
  • 100V, 30min
• Gel Extraction (0.5h)
  • Qiagene GelExtraction Kit
• Ligation (3hr)
  • T4 ligase: 1ul
  • Incubate at RT for 3h
• Plate Culture and Transformation(16h)
  • DNA: 2ul
  • Competent cell: 50ul
• Liquid Culture (16h)
  • 5ml LB
• Plasmid Extraction (1hr)
  • Qiagene Miniprep Kit
• Enzyme digestion to ligation formula
  • phptest