From 2007.igem.org

Revision as of 19:52, 10 September 2007 (view source) Robertina (Talk | contribs) ← Older edit		Latest revision as of 11:36, 23 October 2007 (view source) Diego (Talk | contribs)
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-	Our finally vector will be YCplac22.This is a centromeric plasmid,with trip resistence,it's long 4854pb.	+	Our final vector will be YCplac22,an YC-type (centromeric) shuttle vector.
-	[[Image:YCplac22_map.jpg]]	+
-	Selected features	+	'''Vector Information''':
-		+
-	lac_promoter        142 - 171	+
-	M13_pUC_rev_primer  185 - 207	+	'''Vector type''': plasmid
-		+
-	M13_reverse_primer  206 - 224	+
-	M13_forward20_primer 306 - 290	+	'''Size (kb): ''' 4.854
-	M13_pUC_fwd_primer  321 - 299	+	'''Construct size (kb): ''' 4.854
-		+
-	CEN4                504 - 1356	+
-		+
-	Orf frame 2          2740 - 2066	+
-	TRP1                2740 - 2066	+	'''Construction:''' pUC19
-	AmpR_promoter        2983 - 3011	+	'''Markers:''' TRP1,  ampR
-	Orf frame 2          3053 - 3913	+	'''Features:''' insert detection: lacZ'
-	Ampicillin          3053 - 3913	+	'''marker(s):''' TRP1, ampR
-		+
-	pBR322_origin        4068 - 4687	+
		+	'''promoter:''' lac
-	Unique restriction sites	+	'''replicon:''' ARS1, pMB1
-	HindIII   233	+	'''MCS:''' HindIII...EcoRI
		+
		+	'''centromere:''' CEN4
-	PstI   249	+	[[Image:YCplac22_map.jpg]]
		+
		+	'''Selected features'''
		+
		+	lac_promoter                      142 - 171
		+
		+	M13_pUC_rev_primer                185 - 207
-	SalI  251	+	M13_reverse_primer                206 - 224
		+
		+	M13_forward20_primer              306 - 290
		+
		+	M13_pUC_fwd_primer                321 - 299
-	XbaI  257	+	CEN4                              504 - 1356
-	BamHI  263	+	Orf frame 2                      2740 - 2066
-		+
-	SmaI  270	+	TRP1                              2740 - 2066
-		+
-	KpnI  276	+	AmpR_promoter                    2983 - 3011
-		+
-	SacI  282	+	Orf frame 2                      3053 - 3913
-		+
-	EcoRI  284	+	Ampicillin                        3053 - 3913
-		+
-	NarI  446	+
-		+
-	SpeI  550	+
-		+
-	NheI  1801	+
-		+
-	BglII  1986	+
-		+
-	EcoRV  2455	+
-		+
-	PmlI  2780	+
-	AatII  2921	+	pBR322_origin                    4068 - 4687

---

Latest revision as of 11:36, 23 October 2007

Our final vector will be YCplac22,an YC-type (centromeric) shuttle vector.

Vector Information:

Vector type: plasmid

Size (kb): 4.854

Construct size (kb): 4.854

Construction: pUC19

Markers: TRP1, ampR

Features: insert detection: lacZ'

marker(s): TRP1, ampR

promoter: lac

replicon: ARS1, pMB1

MCS: HindIII...EcoRI

centromere: CEN4

Selected features

lac_promoter 142 - 171

M13_pUC_rev_primer 185 - 207

M13_reverse_primer 206 - 224

M13_forward20_primer 306 - 290

M13_pUC_fwd_primer 321 - 299

CEN4 504 - 1356

Orf frame 2 2740 - 2066

TRP1 2740 - 2066

AmpR_promoter 2983 - 3011

Orf frame 2 3053 - 3913

Ampicillin 3053 - 3913

pBR322_origin 4068 - 4687