Biobrick Vector choice
From 2007.igem.org
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| - | We have selected from BioBrick pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. | + | We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. |
This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance. | This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance. | ||
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Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. | Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. | ||
| - | We | + | We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI. |
[[Image:Gfp-report2.jpg]] [[Image:Rfp-reporter.jpg]] | [[Image:Gfp-report2.jpg]] [[Image:Rfp-reporter.jpg]] | ||
Latest revision as of 11:35, 23 October 2007
We have selected from BioBrick plate the pSB1A3 plasmid to clone 1Xore and 2Xore promoters and pho4 coding sequence. This is a high copy number plasmid (copy number of 100-300 per cell) carrying ampicillin resistance.
Instead we have selected J61002 and J61003 plasmids to clone respective Pho80 and Pho8 coding sequence. We decided to insert a promoter element between EcoRI and XbaI and Pho80 or Ph08 Cds with XbaI and SpeI.
