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-	FOX3 XbaI Fw	+	[[Image:Primers.jpg]]
-	5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’	+
-	Lungh=21	+	'''''FOX3 XbaI Fw'''''
		+
		+	5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
		+
	Tm 60°C		Tm 60°C
-	FOX3 SpeI Rev	+	'''''FOX3 SpeI Rev''
		+
	5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’		5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
-	Lungh=19	+
	Tm 50°C		Tm 50°C
-	LUCIFERASE Xba I  Fv	+	'''''Luciferase Xba I  Fv'''''
		+
	5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’		5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’
-	Lungh=22	+
	Tm 60°C		Tm 60°C
-	LUCIFERASE   Spe I Rv	+	'''''Luciferase   Spe I Rv'' '''
		+
	5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’		5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
-	Lungh=20	+
	Tm 60°C		Tm 60°C
-	2XORE – CYC1 XbaI  Fw	+	'''''2XORE – CYC1 XbaI  Fw'''''
		+
	5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’		5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
-	Lungh=22	+
	Tm 58,4°C		Tm 58,4°C
-	1X ORE – CYC1 XbaI Fw	+	'''''1X ORE – CYC1 XbaI Fw'''''
		+
	5’- GC TCTAGA GAGATGGCTCGACCATCT-3’		5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
-	Lungh=18	+
	Tm 56°C		Tm 56°C
-	2&1ORE-CYC1 Spe I Rev	+	'''''2&1ORE-CYC1 Spe I Rev'''''
		+
	5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’		5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
-	Lungh=18	+
	Tm 56°C		Tm 56°C

---

Latest revision as of 11:20, 21 October 2007

FOX3 XbaI Fw

5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’

Tm 60°C

FOX3 SpeI Rev

5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’

Tm 50°C

Luciferase Xba I Fv

5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’

Tm 60°C

Luciferase Spe I Rv

5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’

Tm 60°C

2XORE – CYC1 XbaI Fw

5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’

Tm 58,4°C

1X ORE – CYC1 XbaI Fw

5’- GC TCTAGA GAGATGGCTCGACCATCT-3’

Tm 56°C

2&1ORE-CYC1 Spe I Rev

5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’

Tm 56°C