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Revision as of 13:33, 20 September 2007 (view source) Robertina (Talk | contribs) ← Older edit		Latest revision as of 11:20, 21 October 2007 (view source) Robertina (Talk | contribs)
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		+	[[Image:Primers.jpg]]
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	'''''FOX3 XbaI Fw'''''		'''''FOX3 XbaI Fw'''''
-	5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’	+	5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
-	Lungh=21	+	Tm 60°C
-	Tm 60°C
-	'''
	'''''FOX3 SpeI Rev''		'''''FOX3 SpeI Rev''
	5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’		5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
-
-	Lungh=19
	Tm 50°C		Tm 50°C
Line 18:		Line 16:
	5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’		5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’
-
-	Lungh=22
	Tm 60°C		Tm 60°C
-	'''''Luciferase  Spe I Rv'' '''	+	'''''Luciferase  Spe I Rv'' '''
	5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’		5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
-
-	Lungh=20
	Tm 60°C		Tm 60°C
Line 34:		Line 28:
	5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’		5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
-
-	Lungh=22
	Tm 58,4°C		Tm 58,4°C
Line 42:		Line 34:
	5’- GC TCTAGA GAGATGGCTCGACCATCT-3’		5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
-
-	Lungh=18
	Tm 56°C		Tm 56°C
Line 50:		Line 40:
	5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’		5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
-
-	Lungh=18
	Tm 56°C		Tm 56°C

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Latest revision as of 11:20, 21 October 2007

FOX3 XbaI Fw

5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’

Tm 60°C

FOX3 SpeI Rev

5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’

Tm 50°C

Luciferase Xba I Fv

5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’

Tm 60°C

Luciferase Spe I Rv

5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’

Tm 60°C

2XORE – CYC1 XbaI Fw

5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’

Tm 58,4°C

1X ORE – CYC1 XbaI Fw

5’- GC TCTAGA GAGATGGCTCGACCATCT-3’

Tm 56°C

2&1ORE-CYC1 Spe I Rev

5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’

Tm 56°C