Biobrick Primers Design
From 2007.igem.org
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'''''FOX3 XbaI Fw''''' | '''''FOX3 XbaI Fw''''' | ||
| - | 5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’ | + | 5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’ |
Tm 60°C | Tm 60°C | ||
| - | + | '''''FOX3 SpeI Rev'' | |
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’ | 5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’ | ||
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Tm 60°C | Tm 60°C | ||
| - | '''''Luciferase Spe I Rv'' ''' | + | '''''Luciferase Spe I Rv'' ''' |
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’ | 5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’ | ||
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Tm 56°C | Tm 56°C | ||
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Latest revision as of 11:20, 21 October 2007
FOX3 XbaI Fw
5’- GC TCTAGA AGTATGTCGTGCTCGAAGTCA -3’
Tm 60°C
FOX3 SpeI Rev
5’- GG ACTAGA TGCTTTTATGTAGTGTTAT-3’
Tm 50°C
Luciferase Xba I Fv
5’- GC TCTAGA ATGGAAGACGCCAAAAACATAA-3’
Tm 60°C
Luciferase Spe I Rv
5’- GG ACTAGA TTAAAGCTTCTTTCCGCCCTT-3’
Tm 60°C
2XORE – CYC1 XbaI Fw
5’-GC TCTAGA ATCAGTTATTACCCTCGACCTC-3’
Tm 58,4°C
1X ORE – CYC1 XbaI Fw
5’- GC TCTAGA GAGATGGCTCGACCATCT-3’
Tm 56°C
2&1ORE-CYC1 Spe I Rev
5’- GG ACTAGA GCCATGGCCCATCGAATTC-3’
Tm 56°C
