Melbourne/Secondary Reagent LB
From 2007.igem.org
< Melbourne(Difference between revisions)
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For 1L: | For 1L: | ||
| - | *10g | + | *10g [[Melbourne/primary Bacto-tryptone|Bacto-tryptone]] |
| - | *5g | + | *5g [[Melbourne/primary Yeast Extract|Yeast Extract]] |
| - | *10g NaCl | + | *10g [[Melbourne/primary Nacl|NaCl]] |
| - | *NaOH to adjust pH | + | *[[Melbourne/primary NaOH|NaOH]] to adjust pH |
===Method:=== | ===Method:=== | ||
#Place Ingredients in a plastic container and add sterile water to the desired volume. | #Place Ingredients in a plastic container and add sterile water to the desired volume. | ||
#Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5. | #Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5. | ||
Latest revision as of 11:02, 29 September 2007
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Contents |
Preparation of LB
Uses
- Culturing Bacteria
- Making LB-Agar Plates
Ingredients:
For 1L:
- 10g Bacto-tryptone
- 5g Yeast Extract
- 10g NaCl
- NaOH to adjust pH
Method:
- Place Ingredients in a plastic container and add sterile water to the desired volume.
- Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.
