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Revision as of 11:02, 29 September 2007 (view source) Craig (Talk | contribs) ← Older edit		Latest revision as of 11:02, 29 September 2007 (view source) Craig (Talk | contribs) (→Ingredients:)
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	*10g [[Melbourne/primary Bacto-tryptone|Bacto-tryptone]]		*10g [[Melbourne/primary Bacto-tryptone|Bacto-tryptone]]
	*5g [[Melbourne/primary Yeast Extract|Yeast Extract]]		*5g [[Melbourne/primary Yeast Extract|Yeast Extract]]
-	*10g[[Melbourne/primary Nacl|NaCl]]	+	*10g [[Melbourne/primary Nacl|NaCl]]
	*[[Melbourne/primary NaOH|NaOH]] to adjust pH		*[[Melbourne/primary NaOH|NaOH]] to adjust pH

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Latest revision as of 11:02, 29 September 2007

<return to list of secondary methods> <team home page>

Contents 1 Preparation of LB 2 Uses 3 Ingredients: 4 Method:

Preparation of LB

Uses

• Culturing Bacteria
• Making LB-Agar Plates

Ingredients:

For 1L:

• 10g Bacto-tryptone
• 5g Yeast Extract
• 10g NaCl
• NaOH to adjust pH

Method:

1. Place Ingredients in a plastic container and add sterile water to the desired volume.
2. Mix using a magnetic stirrer and adjust pH using NaOH until the pH is 7.5.