NYMU Taipei/Lab Notes/2007 10 3
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==GEL check for digestions== | ==GEL check for digestions== | ||
=== System 1 === | === System 1 === | ||
| - | + | * TATA_INSA ('''X.P. digestion, 10/2''') | |
| - | + | * TATB_INSB ('''E.S. digestion, 10/2''') | |
| - | + | * CinR+HSL+D-term ('''E. digestion, 10/2''') | |
| - | + | ||
=== System 2 === | === System 2 === | ||
| + | * OmpRBS ('''X. P. digestion, 10/2''') | ||
| + | [[Image:NYMU Taipei 20071003 TATA INSA,TATB INSB,OmpRBS,TATD IDE digestion check.jpg]] | ||
| + | * lane1 : 1kd ladder | ||
| + | * lane2-3: TATA_INSA (D. XP) | ||
| + | * lane3-4: TATB_INSB (D. ES) | ||
| + | * lane5-6: OmpRBS (D. XP) | ||
| + | * lane7 : TATD_IDE (D. E) | ||
| + | == three digestion for assembly == | ||
| + | * check [https://2007.igem.org/NYMU_Taipei/Lab_Notes/2007_10_2#digestion_for_assembly here] | ||
| + | == next tasks == | ||
| + | * pOmpC + TATA_INSA | ||
| + | * TATB_INSB + CinR+HSL+D-term | ||
* pCinRHSL + OmpRBS | * pCinRHSL + OmpRBS | ||
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Latest revision as of 13:55, 3 October 2007
Contents |
GEL check for digestions
System 1
- TATA_INSA (X.P. digestion, 10/2)
- TATB_INSB (E.S. digestion, 10/2)
- CinR+HSL+D-term (E. digestion, 10/2)
System 2
- OmpRBS (X. P. digestion, 10/2)
- lane1 : 1kd ladder
- lane2-3: TATA_INSA (D. XP)
- lane3-4: TATB_INSB (D. ES)
- lane5-6: OmpRBS (D. XP)
- lane7 : TATD_IDE (D. E)
three digestion for assembly
- check here
next tasks
- pOmpC + TATA_INSA
- TATB_INSB + CinR+HSL+D-term
- pCinRHSL + OmpRBS
