|
|
| (43 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | <br>
| |
| - | <br><br><br><br><br><br><br><br><br><br>
| |
| | | | |
| - | <html>
| |
| - | <head>
| |
| - |
| |
| - |
| |
| - | <style type="text/css">
| |
| - | h1.firstHeading {display: none;}
| |
| - | #navmenu {
| |
| - | float: center;
| |
| - | width: 856px;
| |
| - | position: absolute;
| |
| - | padding: 0;
| |
| - | margin: 0 0 0 0;
| |
| - | list-style: none;
| |
| - | z-index:50;
| |
| - | margin-bottom: 0px;
| |
| - | background-image: white;
| |
| - |
| |
| - | }
| |
| - |
| |
| - | #navmenu ul {
| |
| - | list-style: none;
| |
| - | position:absolute;
| |
| - | border-right: solid 0px #666;
| |
| - | border-bottom: solid 0px #666;
| |
| - | border-left: solid 0px #666;
| |
| - | line-height: 8pt;
| |
| - | }
| |
| - |
| |
| - | #navmenu a {
| |
| - | font-size: 14pt;
| |
| - | font-family: "Lucida Grande", Verdana, arial, sans-serif;
| |
| - | text-decoration: none;
| |
| - | color: #0000FF;
| |
| - | display: block;
| |
| - | }
| |
| - |
| |
| - | #navmenu a:hover {
| |
| - | color: #0000FF;
| |
| - | }
| |
| - |
| |
| - | #navmenu li {
| |
| - | list-style: none;
| |
| - | padding: .3em 1em;
| |
| - | float:left;
| |
| - | background-color: white;
| |
| - | }
| |
| - |
| |
| - | #navmenu li td a:visited { color: #FF0000; }
| |
| - |
| |
| - | #navmenu li ul {
| |
| - | display: none;
| |
| - | margin: 0;
| |
| - | padding-left:0;
| |
| - | background:white;
| |
| - | list-style: none;
| |
| - | }
| |
| - |
| |
| - | #navmenu li:hover ul {
| |
| - | display: block;
| |
| - | list-style: none;
| |
| - | }
| |
| - |
| |
| - | #navmenu li li {
| |
| - | padding: 0.2em 0.5em;
| |
| - | float:none;
| |
| - | }
| |
| - |
| |
| - | #navmenu li li:hover {
| |
| - | background-color: #FFFF00;
| |
| - | }
| |
| - |
| |
| - | #navmenu li ul a {
| |
| - | font-size: 8pt;
| |
| - | color: #000000;
| |
| - | }
| |
| - |
| |
| - | #navmenu #rightLink a{
| |
| - | float:right;
| |
| - | padding:0.2em 0.5em;
| |
| - | color: #FF0000;
| |
| - | }
| |
| - |
| |
| - | #navmenu #rightLink a:hover{
| |
| - | color: #FF0000;
| |
| - | }
| |
| - | .style5 {
| |
| - | font-size: medium;
| |
| - | font-weight: bold;
| |
| - | }
| |
| - | </style>
| |
| - | </style>
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
| |
| - | <title>Untitled Document</title>
| |
| - | <style type="text/css">
| |
| - | <!--
| |
| - | #apDiv1 {
| |
| - | position:absolute;
| |
| - | left:50%;
| |
| - | top:275px;
| |
| - | width:281px;
| |
| - | height:193px;
| |
| - | z-index:1;
| |
| - | }
| |
| - | #apDiv2 {
| |
| - | position:absolute;
| |
| - | left:1px;
| |
| - | top:2px;
| |
| - | width:960px;
| |
| - | height:323px;
| |
| - | z-index:1;
| |
| - | background-color: #FFFFFF;
| |
| - | }
| |
| - | #apDiv3 {
| |
| - | position:absolute;
| |
| - | left:0px;
| |
| - | top:344px;
| |
| - | width:904px;
| |
| - | height:50px;
| |
| - | z-index:2;
| |
| - | }
| |
| - | #apDiv4 {
| |
| - | position:absolute;
| |
| - | left:303px;
| |
| - | top:92px;
| |
| - | width:305px;
| |
| - | height:348px;
| |
| - | z-index:3;
| |
| - | }
| |
| - | #apDiv5 {
| |
| - | position:absolute;
| |
| - | left:3px;
| |
| - | top:0px;
| |
| - | width:101px;
| |
| - | height:1000px;
| |
| - | z-index:4;
| |
| - | }
| |
| - | #apDiv6 {
| |
| - | position:absolute;
| |
| - | left:358px;
| |
| - | top:-1px;
| |
| - | width:75px;
| |
| - | height:93px;
| |
| - | z-index:5;
| |
| - | }
| |
| - | #apDiv7 {
| |
| - | position:absolute;
| |
| - | left:1200px;
| |
| - | top:270px;
| |
| - | width:102px;
| |
| - | height:100px;
| |
| - | z-index:6;
| |
| - | }
| |
| - | #apDiv8 {
| |
| - | position:absolute;
| |
| - | left:202px;
| |
| - | top:393px;
| |
| - | width:1px;
| |
| - | height:0px;
| |
| - | z-index:7;
| |
| - | }
| |
| - | #apDiv9 {
| |
| - | position:absolute;
| |
| - | left:1323px;
| |
| - | top:251px;
| |
| - | width:105px;
| |
| - | height:159px;
| |
| - | z-index:7;
| |
| - | }
| |
| - | #apDiv10 {
| |
| - | position:absolute;
| |
| - | left:1301px;
| |
| - | top:464px;
| |
| - | width:94px;
| |
| - | height:120px;
| |
| - | z-index:9;
| |
| - | background-color: #FFFFFF;
| |
| - | }
| |
| - | #apDiv11 {
| |
| - | position:absolute;
| |
| - | left:211px;
| |
| - | top:130px;
| |
| - | width:84px;
| |
| - | height:91px;
| |
| - | z-index:10;
| |
| - | }
| |
| - | #apDiv12 {
| |
| - | position:absolute;
| |
| - | left:1297px;
| |
| - | top:861px;
| |
| - | width:123px;
| |
| - | height:129px;
| |
| - | z-index:11;
| |
| - | }
| |
| - | #apDiv13 {
| |
| - | position:absolute;
| |
| - | left:576px;
| |
| - | top:-2px;
| |
| - | width:68px;
| |
| - | height:75px;
| |
| - | z-index:12;
| |
| - | }
| |
| - | #apDiv14 {
| |
| - | position:absolute;
| |
| - | left:628px;
| |
| - | top:214px;
| |
| - | width:88px;
| |
| - | height:125px;
| |
| - | z-index:13;
| |
| - | }
| |
| - | #apDiv15 {
| |
| - | position:absolute;
| |
| - | left:937px;
| |
| - | top:762px;
| |
| - | width:107px;
| |
| - | height:128px;
| |
| - | z-index:14;
| |
| - | }
| |
| - | #apDiv16 {
| |
| - | position:absolute;
| |
| - | left:595px;
| |
| - | top:429px;
| |
| - | width:87px;
| |
| - | height:100px;
| |
| - | z-index:15;
| |
| - | }
| |
| - | #apDiv17 {
| |
| - | position:absolute;
| |
| - | left:816px;
| |
| - | top:755px;
| |
| - | width:65px;
| |
| - | height:27px;
| |
| - | z-index:5;
| |
| - | background-color: #FFFFFF;
| |
| - | }
| |
| - | #apDiv18 {
| |
| - | position:absolute;
| |
| - | left:1123px;
| |
| - | top:720px;
| |
| - | width:95px;
| |
| - | height:110px;
| |
| - | z-index:16;
| |
| - | }
| |
| - | #apDiv19 {
| |
| - | position:absolute;
| |
| - | left:254px;
| |
| - | top:397px;
| |
| - | width:86px;
| |
| - | height:105px;
| |
| - | z-index:17;
| |
| - | }
| |
| - | #apDiv20 {
| |
| - | position:absolute;
| |
| - | left:476px;
| |
| - | top:436px;
| |
| - | width:114px;
| |
| - | height:101px;
| |
| - | z-index:18;
| |
| - | }
| |
| - | #apDiv21 {
| |
| - | position:absolute;
| |
| - | left:1114px;
| |
| - | top:564px;
| |
| - | width:87px;
| |
| - | height:109px;
| |
| - | z-index:19;
| |
| - | }
| |
| - | #apDiv22 {
| |
| - | position:absolute;
| |
| - | left:48px;
| |
| - | top:274px;
| |
| - | width:120px;
| |
| - | height:25px;
| |
| - | z-index:30;
| |
| - | }
| |
| - | #apDiv23 {
| |
| - | position:absolute;
| |
| - | left:47px;
| |
| - | top:406px;
| |
| - | width:117px;
| |
| - | height:24px;
| |
| - | z-index:30;
| |
| - | }
| |
| - | #apDiv24 {
| |
| - | position:absolute;
| |
| - | left:48px;
| |
| - | top:537px;
| |
| - | width:116px;
| |
| - | height:22px;
| |
| - | z-index:30;
| |
| - | }
| |
| - | #apDiv25 {
| |
| - | position:absolute;
| |
| - | left:50px;
| |
| - | top:669px;
| |
| - | width:146px;
| |
| - | height:25px;
| |
| - | z-index:30;
| |
| - | }
| |
| - | #apDiv26 {
| |
| - | position:absolute;
| |
| - | left:48px;
| |
| - | top:802px;
| |
| - | width:161px;
| |
| - | height:20px;
| |
| - | z-index:30;
| |
| - | }
| |
| - | #apDiv27 {
| |
| - | position:absolute;
| |
| - | left:49px;
| |
| - | top:934px;
| |
| - | width:149px;
| |
| - | height:22px;
| |
| - | z-index:40;
| |
| - | }
| |
| - | #apDiv28 {
| |
| - | position:absolute;
| |
| - | left:1159px;
| |
| - | top:114px;
| |
| - | width:223px;
| |
| - | height:46px;
| |
| - | z-index:25;
| |
| - | }
| |
| - | #apDiv29 {
| |
| - | position:absolute;
| |
| - | left:15px;
| |
| - | top:594px;
| |
| - | width:149px;
| |
| - | height:32px;
| |
| - | z-index:25;
| |
| - | }
| |
| - | #apDiv30 {
| |
| - | position:absolute;
| |
| - | left:15px;
| |
| - | top:695px;
| |
| - | width:138px;
| |
| - | height:22px;
| |
| - | z-index:26;
| |
| - | }
| |
| - | #apDiv31 {
| |
| - | position:absolute;
| |
| - | left:16px;
| |
| - | top:796px;
| |
| - | width:136px;
| |
| - | height:24px;
| |
| - | z-index:27;
| |
| - | }
| |
| - | #apDiv32 {
| |
| - | position:absolute;
| |
| - | left:17px;
| |
| - | top:898px;
| |
| - | width:139px;
| |
| - | height:22px;
| |
| - | z-index:28;
| |
| - | }
| |
| - | #apDiv33 {
| |
| - | position:absolute;
| |
| - | left:16px;
| |
| - | top:1001px;
| |
| - | width:138px;
| |
| - | height:24px;
| |
| - | z-index:29;
| |
| - | }
| |
| - | #apDiv34 {
| |
| - | position:absolute;
| |
| - | left:16px;
| |
| - | top:1103px;
| |
| - | width:142px;
| |
| - | height:27px;
| |
| - | z-index:30;
| |
| - | }
| |
| - | #apDiv35 {
| |
| - | position:absolute;
| |
| - | left:3px;
| |
| - | top:100px;
| |
| - | width:950px;
| |
| - | height:130px;
| |
| - | z-index:41;
| |
| - | }
| |
| - | body {
| |
| - | background-color: #FFFFFF;
| |
| - | margin-top: 0px;
| |
| - | }
| |
| - | #apDiv36 {
| |
| - | position:absolute;
| |
| - | left:641px;
| |
| - | top:496px;
| |
| - | width:183px;
| |
| - | height:52px;
| |
| - | z-index:31;
| |
| - | }
| |
| - | #apDiv37 {
| |
| - | position:absolute;
| |
| - | left:18px;
| |
| - | top:1197px;
| |
| - | width:50px;
| |
| - | height:54px;
| |
| - | z-index:32;
| |
| - | }
| |
| - | .style2 {font-size: medium}
| |
| - | #apDiv38 {
| |
| - | position:absolute;
| |
| - | left:0px;
| |
| - | top:0px;
| |
| - | width:956px;
| |
| - | height:101px;
| |
| - | z-index:32;
| |
| - | }
| |
| - | #apDiv39 {
| |
| - | position:absolute;
| |
| - | left:0px;
| |
| - | top:201px;
| |
| - | width:956px;
| |
| - | height:81px;
| |
| - | z-index:33;
| |
| - | }
| |
| - | #apDiv40 {
| |
| - | position:absolute;
| |
| - | left:191px;
| |
| - | top:542px;
| |
| - | width:433px;
| |
| - | height:26px;
| |
| - | z-index:34;
| |
| - | }
| |
| - | #apDiv41 {
| |
| - | position:absolute;
| |
| - | left:3px;
| |
| - | top:654px;
| |
| - | width:950px;
| |
| - | height:160px;
| |
| - | z-index:35;
| |
| - | }
| |
| - | #apDiv42 {
| |
| - | position:absolute;
| |
| - | left:15px;
| |
| - | top:602px;
| |
| - | width:163px;
| |
| - | height:29px;
| |
| - | z-index:36;
| |
| - | }
| |
| - | #apDiv43 {
| |
| - | position:absolute;
| |
| - | left:15px;
| |
| - | top:703px;
| |
| - | width:163px;
| |
| - | height:43px;
| |
| - | z-index:37;
| |
| - | }
| |
| - | #apDiv44 {
| |
| - | position:absolute;
| |
| - | left:15px;
| |
| - | top:806px;
| |
| - | width:164px;
| |
| - | height:41px;
| |
| - | z-index:38;
| |
| - | }
| |
| - | #apDiv45 {
| |
| - | position:absolute;
| |
| - | left:24px;
| |
| - | top:907px;
| |
| - | width:155px;
| |
| - | height:41px;
| |
| - | z-index:39;
| |
| - | }
| |
| - | #apDiv46 {
| |
| - | position:absolute;
| |
| - | left:15px;
| |
| - | top:1011px;
| |
| - | width:163px;
| |
| - | height:40px;
| |
| - | z-index:40;
| |
| - | }
| |
| - | #apDiv47 {
| |
| - | position:absolute;
| |
| - | left:22px;
| |
| - | top:1112px;
| |
| - | width:155px;
| |
| - | height:42px;
| |
| - | z-index:41;
| |
| - | }
| |
| - | a:link {
| |
| - | color: #000000;
| |
| - | }
| |
| - | a:visited {
| |
| - | color: #000000;
| |
| - | }
| |
| - | a:hover {
| |
| - | color: #FF0000;
| |
| - | }
| |
| - | a:active {
| |
| - | color: #3300FF;
| |
| - | }
| |
| - | #apDiv48 {
| |
| - | position:absolute;
| |
| - | left:145px;
| |
| - | top:1px;
| |
| - | width:96px;
| |
| - | height:72px;
| |
| - | z-index:42;
| |
| - | }
| |
| - | #apDiv49 {
| |
| - | position:absolute;
| |
| - | left:0px;
| |
| - | top:-20px;
| |
| - | width:100px;
| |
| - | height:85px;
| |
| - | z-index:43;
| |
| - | }
| |
| - | #apDiv50 {
| |
| - | position:absolute;
| |
| - | left:1px;
| |
| - | top:0px;
| |
| - | width:136px;
| |
| - | height:531px;
| |
| - | z-index:43;
| |
| - | }
| |
| - | .style12 {
| |
| - | font-size: small;
| |
| - | font-weight: bold;
| |
| - | }
| |
| - | .style13 {font-size: small}
| |
| - | -->
| |
| - | </style>
| |
| - | </head>
| |
| - |
| |
| - | <body>
| |
| - | <div id="apDiv4"><img src="https://static.igem.org/mediawiki/2007/8/8e/Red_Bacterium_With_Link_LinesV5.gif" alt="" name="RedBacterium" width="332" height="342" id="RedBacterium" /></div>
| |
| - | <div id="apDiv6">
| |
| - | <div align="center"> <span class="style2"><strong><a href="https://2007.igem.org/Berkeley_UC_Hemoglobin">Oxygen Transport</a></strong></span><br>
| |
| - | <a href="https://2007.igem.org/Berkeley_UC_Hemoglobin"><img name="HemoglobinButton" src="https://static.igem.org/mediawiki/2007/c/cf/HemoglobinButton.gif" width="75" height="75" alt=""></a><br>
| |
| - | </div>
| |
| - | </div>
| |
| - | <div id="apDiv11">
| |
| - | <div align="center"><strong><span class="style2"><a href="https://2007.igem.org/Berkeley_UC_Chassis"> Immunity</a> </span><br>
| |
| - | </strong><a href="https://2007.igem.org/Berkeley_UC_msbB"><img name="ImmunityButton" src="https://static.igem.org/mediawiki/2007/f/f9/ImmunityButton.gif" width="100" height="75" alt=""></a></div>
| |
| - | </div>
| |
| - | <div id="apDiv13">
| |
| - | <div align="center">
| |
| - | <p><span class="style2"><strong><a href="https://2007.igem.org/Berkeley_UC_Storage">Storage<br>
| |
| - | </a></strong></span><a href="https://2007.igem.org/Berkeley_UC_Storage"><img name="TrehaloseButton" src="https://static.igem.org/mediawiki/2007/7/7a/TrehaloseButton.gif" width="75" height="75" alt=""></a></p>
| |
| - | </div>
| |
| - | </div>
| |
| - | <div id="apDiv14">
| |
| - | <div align="center"><span class="style5"><a href="https://2007.igem.org/Berkeley_UC_SystemController">System Controller</a></span><a href="https://2007.igem.org/Berkeley_UC_SystemController"><img name="ControllerButton" src="https://static.igem.org/mediawiki/2007/c/cd/2PlasmidButton.gif" width="75" height="75" alt=""></a></div>
| |
| - | </div>
| |
| - | <div id="apDiv16">
| |
| - | <div align="center"><span class="style5"><a href="https://2007.igem.org/Berkeley_UC_KillSwitch">Kill Switch</a></span><a href="https://2007.igem.org/Berkeley_UC_KillSwitch"><img name="KillSwitchButton" src="https://static.igem.org/mediawiki/2007/f/ff/KillSwitchButton.gif" width="75" height="75" alt=""></a></div>
| |
| - | </div>
| |
| - | <div id="apDiv19">
| |
| - | <div align="center" class="style5"><a href="https://2007.igem.org/Berkeley_UC_Antioxidants">Antioxidants</a></div>
| |
| - | <div align="center"><a href="https://2007.igem.org/Berkeley_UC_Antioxidants"><img name="SODButton" src="https://static.igem.org/mediawiki/2007/4/45/SODButton.gif" width="75" height="75" alt=""></a></div>
| |
| - | </div>
| |
| - | <div id="apDiv20">
| |
| - | <div align="center"><span class="style5"><a href="https://2007.igem.org/Berkeley_UC_Deletions">Growth Control</a></span><a href="https://2007.igem.org/Berkeley_UC_Deletions"><img name="GrowthControlButton" src="https://static.igem.org/mediawiki/2007/b/bf/DNADeletionButton.gif" width="75" height="75" alt=""></a></div>
| |
| - | </div>
| |
| - | <div id="apDiv36">
| |
| - | <div align="center"><span class="style5"><a href="https://2007.igem.org/Berkeley_UC_Hemoglobin">Begin Project Tour</a></span><a href="https://2007.igem.org/Berkeley_UC_Hemoglobin"><img name="UCForwardButton" src="https://static.igem.org/mediawiki/2007/a/a0/F-Blue.gif" width="65" height="50" alt=""></a></div>
| |
| - | </div>
| |
| - |
| |
| - | <div id="apDiv40">Best viewed in the <a href="http://www.mozilla.com/en-US/firefox/"><strong>most recent version of firefox</strong></a>, because IE sucks. </div>
| |
| - | <div id="apDiv41"><span class="style2"><strong>The global demand and importance</strong> for cheap, available, and disease free blood substitutes is undisputed. There are currently no red blood cell substitutes approved for clinical use in the US or the UK, and whole blood is almost always in short supply. Underdeveloped countries that need blood the most simply don’t have the infrastructure to support donation and storage, in addition a sizeable fraction of the population are disease carriers. We are developing an innovative and cheap blood substitute based on engineered <em>E. coli</em> with all the critical properties of human erythrocytes. These include the ability to safely exist in the bloodstream, carry oxygen, and be stored for prolonged periods in a freeze-dried state.</span></div>
| |
| - | <div id="apDiv48"><img name="BloodyiGEMLogo" src="https://static.igem.org/mediawiki/2007/1/10/MovingiGEMlogo.gif" width="95" height="70" alt=""></div>
| |
| - | <div class="style12" id="apDiv50">
| |
| - | <p>Introduction</p>
| |
| - | <p>Oxygen Transport
| |
| - | </p>
| |
| - | <ul>
| |
| - | <li><a href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Hemoglobin" class="style13">Hemoglobin</a></li>
| |
| - | <li><a href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones">Chaperones
| |
| - | </a>
| |
| - | <ul>
| |
| - | <li><a href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_Heme" class="style13">Heme</a></li>
| |
| - | <li><a href="https://2007.igem.org/Berkeley_UC_OxygenTransport_Chaperones_AHSP" class="style13">AHSP<br>
| |
| - | </a> </li>
| |
| - | </ul>
| |
| - | </li>
| |
| - | </ul>
| |
| - | <p class="style13">Immunity</p>
| |
| - | <ul>
| |
| - | <li>Chassis</li>
| |
| - | <li>msbB Deletion</li>
| |
| - | </ul>
| |
| - | <p class="style13">Antioxidants</p>
| |
| - | <ul>
| |
| - | <li>SodC/KatG</li>
| |
| - | </ul>
| |
| - | <p class="style13">Growth Control</p>
| |
| - | <ul>
| |
| - | <li>tonB Deletion</li>
| |
| - | <li>dapD Deletion</li>
| |
| - | </ul>
| |
| - | <p class="style13"><a href="https://2007.igem.org/Berkeley_UC_KillSwitch">Kill Switch</a></p>
| |
| - | <p class="style13"><a href="https://2007.igem.org/Berkeley_UC_SystemController">SystemController</a></p>
| |
| - | <p class="style13"><a href="https://2007.igem.org/Berkeley_UC_Storage">Storage</a></p>
| |
| - | </div>
| |
| - | </body>
| |
| - | </html>
| |
| - | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
| |
| - | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
| |
