BerkiGEM2007-SamanthaConstructionFile1
From 2007.igem.org
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'''[rbs][ATG]''' <br> | '''[rbs][ATG]''' <br> | ||
+ | '''4 Different rbs variants (1106A, 1106B, 1128A, 1090)''' <br> | ||
- | Digest pBca1101- | + | ---- |
- | Digest pBca9145-Bca1112 ( | + | |
+ | |||
+ | Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br> | ||
+ | Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br> | ||
Product is pBca9145-I716201. <br> | Product is pBca9145-I716201. <br> | ||
+ | |||
+ | |||
+ | Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large) <br> | ||
+ | Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br> | ||
+ | Product is pBca9145-I716202. <br> | ||
+ | |||
+ | |||
+ | Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large) <br> | ||
+ | Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br> | ||
+ | Product is pBca9145-I716203. <br> | ||
+ | |||
+ | |||
+ | Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large) <br> | ||
+ | Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small) <br> | ||
+ | Product is pBca9145-I716204. <br> |
Latest revision as of 18:27, 4 June 2007
[rbs][ATG]
4 Different rbs variants (1106A, 1106B, 1128A, 1090)
Digest pBca1101-Bca1106A (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716201.
Digest pBca1101-Bca1106B (BamHI/AlwnI/Xhol, 1588+850+544bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716202.
Digest pBca1101-Bca1128A (BamHI/AlwnI/Xhol, 1606+850+544bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716203.
Digest pBca9145-Bca1090 (BamHI/AlwnI/Xhol, 1538+553+9bp, Large)
Digest pBca9145-Bca1112 (BglII/AlwnI, 1519+562bp, Small)
Product is pBca9145-I716204.