BerkiGEM2007-DavidConstructionFile103
From 2007.igem.org
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PCR T7rnap-FA and T7rnap-R on pBAC-T7621 (2683 bp, BglII/XhoI, Product = A) | PCR T7rnap-FA and T7rnap-R on pBAC-T7621 (2683 bp, BglII/XhoI, Product = A) | ||
PCR T7rnap-FG and T7rnap-R on pBAC-T7621 (2683 bp, BglII/XhoI, Product = G) | PCR T7rnap-FG and T7rnap-R on pBAC-T7621 (2683 bp, BglII/XhoI, Product = G) | ||
| - | Sub | + | Sub each separately into pBca9145-Bca1144 (BglII/XhoI, 2063+910, L) |
| - | Product A is pBca9145-I716103-T7rnap | + | Product A is pBca9145-I716103-T7rnap-A |
| - | Product G is pBca9145-I716104-T7rnap | + | Product G is pBca9145-I716104-T7rnap-G |
--------------------------------------------------- | --------------------------------------------------- | ||
| - | dt005-T7rnap-FA Forward Cloning of T7rnap ATG ccaaaAGATCTatgaacacgattaacatcgc | + | dt005-T7rnap-FA Forward Cloning of T7rnap ATG Start ccaaaAGATCTatgaacacgattaacatcgc |
| - | dt006-T7rnap-FG Forward Cloning of T7rnap GTG ccaaaAGATCTgtgaacacgattaacatcgc | + | dt006-T7rnap-FG Forward Cloning of T7rnap GTG Start ccaaaAGATCTgtgaacacgattaacatcgc |
| - | dt007-T7rnap-R Reverse Cloning of T7rnap | + | dt007-T7rnap-R Reverse Cloning of T7rnap gctagCTCGAGttaGGATCCttacgcgaacgcgaagtccg |
</pre> | </pre> | ||
Latest revision as of 04:44, 8 June 2007
Biobrickificreation of T7rnap I716103, I716104 (T7 RNA Polymerase: ATG, GTG) --------------------------------------------------- PCR T7rnap-FA and T7rnap-R on pBAC-T7621 (2683 bp, BglII/XhoI, Product = A) PCR T7rnap-FG and T7rnap-R on pBAC-T7621 (2683 bp, BglII/XhoI, Product = G) Sub each separately into pBca9145-Bca1144 (BglII/XhoI, 2063+910, L) Product A is pBca9145-I716103-T7rnap-A Product G is pBca9145-I716104-T7rnap-G --------------------------------------------------- dt005-T7rnap-FA Forward Cloning of T7rnap ATG Start ccaaaAGATCTatgaacacgattaacatcgc dt006-T7rnap-FG Forward Cloning of T7rnap GTG Start ccaaaAGATCTgtgaacacgattaacatcgc dt007-T7rnap-R Reverse Cloning of T7rnap gctagCTCGAGttaGGATCCttacgcgaacgcgaagtccg
